Abstract The Protein Data Bank (PDB)––the single global repository of experimentally
determined 3D structures of biological macromolecules and their complexes––was …

Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Public archiving in structural biology is well established with the Protein Data Bank (PDB;
wwPDB. org) catering for atomic models and the Electron Microscopy Data Bank (EMDB; …

The SARS-CoV-2 spike employs mobile receptor-binding domains (RBDs) to engage the
human ACE2 receptor and to facilitate virus entry, which can occur through low-pH …

Multipass membrane proteins play numerous roles in biology and include receptors,
transporters, ion channels and enzymes,. How multipass proteins are co-translationally …

This article describes the implementation of real-space refinement in the phenix.
real_space_refine program from the PHENIX suite. The use of a simplified refinement target …

Abstract The Protein Data Bank (PDB) is the single global archive of experimentally
determined three-dimensional (3D) structure data of biological macromolecules. Since …

Single-particle electron cryomicroscopy (cryo-EM) is a powerful method for determining the
structures of biological macromolecules. With automated microscopes, cryo-EM data can …

Recent advances in the field of electron cryomicroscopy (cryo-EM) have resulted in a rapidly
increasing number of atomic models of biomacromolecules that have been solved using this …

Maintaining a highly acidic lysosomal pH is central to cellular physiology. Here, we use
functional proteomics, single-particle cryo-EM, electrophysiology, and in vivo imaging to …