Clinical Microbiome Analysis by Mass Spectrometry–Based Metaproteomics

X Zhang, Z Ning, J Mayne… - Annual Review of …, 2025 - annualreviews.org
Mass spectrometry–based proteomics and metaproteomics have long been used in the
study of human microbiomes, with the potential of metaproteomics only recently being fully …

The astounding exhaustiveness and speed of the Astral mass analyzer for highly complex samples is a quantum leap in the functional analysis of microbiomes

T Dumas, R Martinez Pinna, C Lozano, S Radau… - Microbiome, 2024 - Springer
Background By analyzing the proteins which are the workhorses of biological systems,
metaproteomics allows us to list the taxa present in any microbiota, monitor their relative …

Construction of Protein Sequence Databases for Metaproteomics: A Review of the Current Tools and Databases

M Arıkan, B Atabay - Journal of Proteome Research, 2024 - ACS Publications
In metaproteomics studies, constructing a reference protein sequence database that is both
comprehensive and not overly large is critical for the peptide identification step. Therefore …

The landscape and perspectives of the human gut metaproteomics

Z Sun, Z Ning, D Figeys - Molecular & Cellular Proteomics, 2024 - ASBMB
The human gut microbiome is closely associated with human health and diseases.
Metaproteomics has emerged as a valuable tool for studying the functionality of the gut …

Data-independent acquisition in Metaproteomics

E Wu, G Xu, D **e, L Qiao - Expert Review of Proteomics, 2024 - Taylor & Francis
Introduction Metaproteomics offers insights into the function of complex microbial
communities, while it is also capable of revealing microbe–microbe and host–microbe …

Spectral entropy as a measure of the metaproteome complexity

H Duan, Z Ning, A Zhang, D Figeys - Proteomics, 2024 - Wiley Online Library
The diversity and complexity of the microbiome's genomic landscape are not always
mirrored in its proteomic profile. Despite the anticipated proteomic diversity, observed …

MetaDIA: A Novel Database Reduction Strategy for DIA Human Gut Metaproteomics

H Duan, Z Ning, Z Sun, T Guo, Y Sun, D Figeys - bioRxiv, 2024 - biorxiv.org
Background Microbiomes, especially within the gut, are complex and may comprise
hundreds of species. The identification of peptides in metaproteomics presents a significant …

MetaLab Platform Enables Comprehensive DDA and DIA Metaproteomics Analysis

K Cheng, Z Ning, X Zhang, H Duan, J Mayne, D Figeys - bioRxiv, 2024 - biorxiv.org
Metaproteomics studies the collective protein composition of complex microbial
communities, providing insights into microbial roles in various environments. Despite its …

Shining a Light on the Dark-Field of the Metaproteome

H Duan - 2024 - ruor.uottawa.ca
Metaproteomics has emerged as a powerful tool for studying human gut microbiomes.
However, the intricate nature of these microbial communities often surpasses the analytical …