Organization and regulation of gene transcription

P Cramer - Nature, 2019 - nature.com
The regulated transcription of genes determines cell identity and function. Recent structural
studies have elucidated mechanisms that govern the regulation of transcription by RNA …

The Paf1 complex: a keystone of nuclear regulation operating at the interface of transcription and chromatin

AM Francette, SA Tripplehorn, KM Arndt - Journal of molecular biology, 2021 - Elsevier
The regulation of transcription by RNA polymerase II is closely intertwined with the
regulation of chromatin structure. A host of proteins required for the disassembly …

RNA polymerase II clustering through carboxy-terminal domain phase separation

M Boehning, C Dugast-Darzacq, M Rankovic… - Nature structural & …, 2018 - nature.com
The carboxy-terminal domain (CTD) of RNA polymerase (Pol) II is an intrinsically disordered
low-complexity region that is critical for pre-mRNA transcription and processing. The CTD …

Structure of activated transcription complex Pol II–DSIF–PAF–SPT6

SM Vos, L Farnung, M Boehning, C Wigge, A Linden… - Nature, 2018 - nature.com
Gene regulation involves activation of RNA polymerase II (Pol II) that is paused and bound
by the protein complexes DRB sensitivity-inducing factor (DSIF) and negative elongation …

Emerging insights into the roles of the Paf1 complex in gene regulation

SB Van Oss, CE Cucinotta, KM Arndt - Trends in biochemical sciences, 2017 - cell.com
The conserved, multifunctional Polymerase-Associated Factor 1 complex (Paf1C) regulates
all stages of the RNA polymerase (Pol) II transcription cycle. In this review, we examine a …

Transcription and splicing: A two‐way street

M Tellier, I Maudlin, S Murphy - Wiley Interdisciplinary Reviews …, 2020 - Wiley Online Library
RNA synthesis by RNA polymerase II and RNA processing are closely coupled during the
transcription cycle of protein‐coding genes. This coupling affords opportunities for quality …

Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells

C Cruz, M Della Rosa, C Krueger, Q Gao, D Horkai… - Elife, 2018 - elifesciences.org
Transcription of protein coding genes is accompanied by recruitment of COMPASS to
promoter-proximal chromatin, which methylates histone H3 lysine 4 (H3K4) to form …

ARTP/EMS-combined multiple mutagenesis efficiently improved production of raw starch-degrading enzymes in Penicillium oxalicum and characterization of the …

LS Gu, MZ Tan, SH Li, T Zhang, QQ Zhang, CX Li… - Biotechnology for …, 2020 - Springer
Background Application of raw starch-degrading enzymes (RSDEs) in starch processing for
biofuel production can effectively reduce energy consumption and processing costs. RSDEs …

The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS

HJ Bae, M Dubarry, J Jeon, LM Soares… - Nature …, 2020 - nature.com
Methylation of histone H3 lysine 4 (H3K4) by Set1/COMPASS occurs co-transcriptionally,
and is important for gene regulation. Set1/COMPASS associates with the RNA polymerase II …

Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteins

C Asencio, A Chatterjee, MW Hentze - Life science alliance, 2018 - life-science-alliance.org
Proteins interact with nucleic acids to regulate cellular functions. The study of these
regulatory interactions is often hampered by the limited efficiency of current protocols to …