Raman microspectroscopy offers microbiologists a rapid and non-destructive technique to
assess the chemical composition of individual live microorganisms in near real time. In this …

Optical microscopes today have pushed the limits of speed, quality, and observable space in
biological specimens revolutionizing how we view life today. Further, specific labeling of …

The advent of image-activated cell sorting and imaging-based cell picking has advanced our
knowledge and exploitation of biological systems in the last decade. Unfortunately, they …

Coherent Raman microscopy is the method of choice for the label-free, real-time
characterization of the chemical composition in biomedical samples. The common …

Metabolic reprogramming in a subpopulation of cancer cells is a hallmark of tumor
chemoresistance. However, single-cell metabolic profiling is difficult because of the lack of a …

Intracellular lipid accumulation is commonly seen in fibrotic livers, but its exact role in liver
fibrosis remains elusive. Here, we established a multimodal nonlinear optical microscopy to …

Rapid antimicrobial susceptibility testing (AST) is urgently needed for treating infections with
appropriate antibiotics and slowing down the emergence of antibiotic‐resistant bacteria …

Raman scattering has long been used to analyze chemical compositions in biological
systems. Owing to its high chemical specificity and noninvasive detection capability, Raman …

Single-cell analysis provides insights into cellular heterogeneity and dynamics of individual
cells. This Feature highlights recent developments in key analytical techniques suited for …

One of the biggest challenges in microbiome research in environmental and medical
samples is to better understand functional properties of microbial community members at a …