The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

The present review offers an overview of nonclassical (eg, with no pre-or in situ activation of
a carboxylic acid partner) approaches for the construction of amide bonds. The review aims …

Today, Fmoc SPPS is the method of choice for peptide synthesis. Very‐high‐quality Fmoc
building blocks are available at low cost because of the economies of scale arising from …

Bioconjugation methodologies have proven to play a central enabling role in the recent
development of biotherapeutics and chemical biology approaches. Recent endeavours in …

Methods to prepare proteins that include a specific modification at a desired position are
essential for understanding their cellular functions and physical properties in living systems …

Contemporary chemical protein synthesis has been dramatically advanced over the past few
decades, which has enabled chemists to reach the landscape of synthetic …

A new record: The largest chemically synthesized polypeptide composed of 304 residues,
which corresponds to folded K48-tetraubiquitin, has been achieved (see scheme). The …

Using a recently discovered peptide ligase, butelase 1, we developed a novel method to
access protein thioesters in good yield. We successfully combined it with native chemical …

Solid-phase peptide synthesis (SPPS) is the preferred strategy for synthesizing most
peptides for research purposes and on a multi-kilogram scale. One key to the success of …

Inteins catalyze a post-translational modification known as protein splicing, where the intein
removes itself from a precursor protein and concomitantly ligates the flanking protein …