Abstract The Ser/Thr kinase mechanistic target of rapamycin (mTOR) is a central regulator of
cellular metabolism. As part of mTOR complex 1 (mTORC1), mTOR integrates signals such …

Since Hedin's characterization of trypsin and antitrypsin in 1906,(1) arguably the first
account of a regulatory protein–protein interaction (PPI), contemporary understanding of …

Although the immediate receptors (immunophilins) of the immunosuppressants cyclosporin
A (CsA) and FK506 are distinct, their similar mechanisms of inhibition of cell signaling …

In the cell, as in vitro, the final conformation of a protein is determined by its amino-acid
sequence. But whereas some isolated proteins can be denatured and refolded in vitro in the …

A nuclear magnetic resonance (NMR)-based method is described in which small organic
molecules that bind to proximal subsites of a protein are identified, optimized, and linked …

CsA and FK506 are powerful suppressors of the immune system, most notably of T cells.
They act at a point in activation that lies between receptor ligation and the transcription of …

Organic molecules as well as metal complexes may exist as several geometric isomers1
which display distinct physical properties and chemical reactivities. A molecule containing …

Rapamycin, a potent immunosuppressive agent, binds two proteins: the FK506-binding
protein (FKBP12) and the FKBP-rapamycin-associated protein (FRAP). A crystal structure of …

A new simple empirical function has been developed that estimates the free energy of
binding for a given protein-ligand complex of known 3D structure. The function takes into …

Dimerization and oligomerization are general biological control mechanisms contributing to
the activation of cell membrane receptors, transcription factors, vesicle fusion proteins, and …