Type III secretion in Chlamydia

EA Rucks - Microbiology and Molecular Biology Reviews, 2023 - Am Soc Microbiol
Type III secretion systems (T3SSs) are utilized by Gram-negative pathogens to enhance
their pathogenesis. This secretion system is associated with the delivery of effectors through …

Cell-free co-translational approaches for producing mammalian receptors: expanding the cell-free expression toolbox using nanolipoproteins

ML Shelby, W He, AT Dang, TL Kuhl… - Frontiers in …, 2019 - frontiersin.org
Membranes proteins make up more than 60% of current drug targets and account for
approximately 30% or more of the cellular proteome. Access to this important class of …

Cell-free production of a functional oligomeric form of a Chlamydia major outer-membrane protein (MOMP) for vaccine development

W He, M Felderman, AC Evans, J Geng… - Journal of Biological …, 2017 - ASBMB
Chlamydia is a prevalent sexually transmitted disease that infects more than 100 million
people worldwide. Although most individuals infected with Chlamydia trachomatis are …

Delivering the pain: an overview of the type III secretion system with special consideration for aquatic pathogens

H Rahmatelahi, M El-Matbouli… - Veterinary …, 2021 - Springer
Gram-negative bacteria are known to subvert eukaryotic cell physiological mechanisms
using a wide array of virulence factors, among which the type three-secretion system (T3SS) …

Cationic HDL mimetics enhance in vivo delivery of self-replicating mRNA

W He, AC Evans, A Rasley, F Bourguet, S Peters… - … , Biology and Medicine, 2020 - Elsevier
In vivo delivery of large RNA molecules has significant implications for novel gene therapy,
biologics delivery, and vaccine applications. We have developed cationic nanolipoprotein …

Macrophage innate immune responses delineate between defective translocon assemblies produced by Yersinia pseudotuberculosis YopD mutants

SI Farag, MK Francis, JM Gurung, SN Wai, H Stenlund… - Virulence, 2023 - Taylor & Francis
Translocon pores formed in the eukaryotic cell membrane by a type III secretion system
facilitate the translocation of immune-modulatory effector proteins into the host cell interior …

Single-molecule fluorescence detection of the epidermal growth factor receptor in membrane discs

SD Quinn, S Srinivasan, JB Gordon, W He… - Biochemistry, 2018 - ACS Publications
The epidermal growth factor receptor (EGFR) is critical to normal cellular signaling
pathways. Moreover, it has been implicated in a range of pathologies, including cancer. As a …

Bimolecular fluorescence complementation assay to explore protein-protein interactions of the Yersinia virulence factor YopM

Ö Uğurlu, S Evran - Biochemical and Biophysical Research …, 2021 - Elsevier
Yersinia outer protein M (YopM) is one of the effector proteins and essential for virulence.
YopM is delivered by the Yersinia type III secretion system (T3SS) into the host cell, where it …

Methods and systems for producing nanolipoprotein particles

MA Coleman, PD Hoeprich, BW Segelke - US Patent 11,300,572, 2022 - Google Patents
2018-12-17 Assigned to LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
reassignment LAWRENCE LIVERMORE NATIONAL SECURITY, LLC ASSIGNMENT OF …

Cell-free scaled production and adjuvant addition to a recombinant major outer membrane protein from chlamydia muridarum for vaccine development

SF Gilmore, W He, AC Evans, DF Tifrea… - Journal of …, 2022 - pmc.ncbi.nlm.nih.gov
Subunit vaccines offer advantages over more traditional inactivated or attenuated whole-cell-
derived vaccines in safety, stability, and standard manufacturing. To achieve an effective …