This Review focuses on how to use DNA nanostructures as scaffolds to organize biological
molecules. First, we introduce the use of structural DNA nanotechnology to engineer …

In prokaryotes, translation can occur on mRNA that is being transcribed in a process called
coupling. How the ribosome affects the RNA polymerase (RNAP) during coupling is not well …

Sortases are enzymes occurring in the cell wall of Gram-positive bacteria. Sortase A (SrtA),
the best studied sortase class, plays a key role in anchoring surface proteins with the …

Creation of functional protein bioconjugates demands methods for attaching a diverse array
of probes to target proteins with high specificity, under mild conditions. The sortase A …

DNA serves as nature's information storage molecule, and has been the primary focus of
engineered systems for biological computing and data storage. Here we combine recent …

We introduce a nanoscale experimental platform that enables kinetic and equilibrium
measurements of a wide range of molecular interactions using a gel electrophoresis …

Immune cells sense, communicate, and logically integrate a multitude of environmental
signals to make important cell‐fate decisions and fulfill their effector functions. These …

Nucleic acids and proteins are two major classes of biopolymers in living systems. Whereas
nucleic acids are characterized by robust molecular recognition properties, essential for the …

Sortase‐mediated ligation (sortagging) is a versatile, powerful strategy for protein
modification. Because the sortase reaction reaches equilibrium, a large excess of …

Scaffold‐based lattice‐engineered 3D DNA origami is a powerful and versatile technique for
the rational design and build‐up of arbitrarily structured and monodisperse DNA‐based 3D …