Bacterial vivisection: how fluorescence-based imaging techniques shed a light on the inner workings of bacteria

A Cambré, A Aertsen - Microbiology and Molecular Biology …, 2020 - Am Soc Microbiol
The rise in fluorescence-based imaging techniques over the past 3 decades has improved
the ability of researchers to scrutinize live cell biology at increased spatial and temporal …

Senescence in bacteria and its underlying mechanisms

UK Steiner - Frontiers in Cell and Developmental Biology, 2021 - frontiersin.org
Bacteria have been thought to flee senescence by dividing into two identical daughter cells,
but this notion of immortality has changed over the last two decades. Asymmetry between …

[HTML][HTML] Protein aggregates encode epigenetic memory of stressful encounters in individual Escherichia coli cells

SK Govers, J Mortier, A Adam, A Aertsen - PLoS biology, 2018 - journals.plos.org
Protein misfolding and aggregation are typically perceived as inevitable and detrimental
processes tied to a stress-or age-associated decline in cellular proteostasis. A careful …

Fast pathogen identification using single-cell matrix-assisted laser desorption/ionization-aerosol time-of-flight mass spectrometry data and deep learning methods

C Papagiannopoulou, R Parchen, P Rubbens… - Analytical …, 2020 - ACS Publications
In diagnostics of infectious diseases, matrix-assisted laser desorption/ionization-time-of-
flight mass spectrometry (MALDI-TOF MS) can be applied for the identification of pathogenic …

Coculturing bacteria leads to reduced phenotypic heterogeneities

J Heyse, B Buysschaert, R Props… - Applied and …, 2019 - Am Soc Microbiol
Isogenic bacterial populations are known to exhibit phenotypic heterogeneity at the single-
cell level. Because of difficulties in assessing the phenotypic heterogeneity of a single taxon …

Gene regulation in Escherichiacoli is commonly selected for both high plasticity and low noise

M Vlková, OK Silander - Nature Ecology & Evolution, 2022 - nature.com
Bacteria often respond to dynamically changing environments by regulating gene
expression. Despite this regulation being critically important for growth and survival, little is …

Stochastic response of bacterial cells to antibiotics: its mechanisms and implications for population and evolutionary dynamics

T Akiyama, M Kim - Current Opinion in Microbiology, 2021 - Elsevier
Highlights•Genetically identical cells exhibit different levels of antibiotic susceptibility.•
Heterogeneous susceptibility (HS) is due to stochastic molecular processes.•HS leads to …

Cancer cell fitness is dynamic

LS Lenz, JL Faccioni, PA Bracco, JAF Santos… - Cancer Research, 2021 - AACR
Several phenotypes that impact the capacity of cancer cells to survive and proliferate are
dynamic. Here we used the number of cells in colonies as an assessment of fitness and …

The role of memory in non-genetic inheritance and its impact on cancer treatment resistance

T Cassidy, D Nichol, M Robertson-Tessi… - PLOS Computational …, 2021 - journals.plos.org
Intra-tumour heterogeneity is a leading cause of treatment failure and disease progression
in cancer. While genetic mutations have long been accepted as a primary mechanism of …

Non-genetic inheritance restraint of cell-to-cell variation

H Vashistha, M Kohram, H Salman - Elife, 2021 - elifesciences.org
Heterogeneity in physical and functional characteristics of cells (eg size, cycle time, growth
rate, protein concentration) proliferates within an isogenic population due to stochasticity in …