Since the first use of Chinese hamster ovary (CHO) cells for recombinant protein expression,
production processes have steadily improved through numerous advances. In this review …

The culture of Chinese Hamster Ovary (CHO) cells for modern industrial applications, such
as expression of recombinant proteins, requires media that support growth and production …

Chinese hamster ovary (CHO) cells dominate biotherapeutic protein production and are
widely used in mammalian cell line engineering research. To elucidate metabolic …

Genome-scale models of metabolism can illuminate the molecular basis of cell phenotypes.
Since some enzymes are only active in specific cell types, several algorithms use omics data …

Chinese hamster ovary (CHO) cells are the main platform for production of biotherapeutics
in the biopharmaceutical industry. However, relatively little is known about the metabolism of …

Ensuring consistent high yields and product quality are key challenges in biomanufacturing.
Even minor deviations in critical process parameters (CPPs) such as media and feed …

The increasing demand for recombinant therapeutic proteins highlights the need to
constantly improve the efficiency and yield of these biopharmaceutical products from …

Mammalian cell culture metabolism is characterized by glucoglutaminolysis, that is, high
glucose and glutamine uptake combined with a high rate of lactate and non-essential amino …

Normally, the growth profile of a CHO cell fed-batch process can be divided into two main
phases based on changes in cell concentration, being an exponential growth phase and a …

Fed-batch cultures are extensively used for the production of therapeutic proteins. However,
process optimization is hampered by lack of quantitative models of mammalian cellular …