A comprehensive review on recent preparation techniques of liposomes

C Has, P Sunthar - Journal of liposome research, 2020 - Taylor & Francis
Liposomes (or lipid vesicles) are a versatile platform as carriers for the delivery of the drugs
and other macromolecules into human and animal bodies. Though the method of using …

Vesicle formation mechanisms: an overview

C Has, S Pan - Journal of liposome research, 2021 - Taylor & Francis
Vesicle structures primarily embody spherical capsules composed of a single or multiple
bilayers, entrap** a pool of aqueous solution in their interior. The bilayers can be …

Continuous flow production of cationic liposomes at high lipid concentration in microfluidic devices for gene delivery applications

TA Balbino, NT Aoki, AAM Gasperini… - Chemical engineering …, 2013 - Elsevier
Microfluidics is a powerful technology that allows the production of cationic liposomes by the
hydrodynamic focusing method. We first studied a single hydrodynamic focusing (SHF) …

[HTML][HTML] Interaction proteomics analysis to provide insight into TFAMoplex-mediated transfection

S Honrath, D Scherer, M Burger, JC Leroux - Journal of Controlled Release, 2024 - Elsevier
In an earlier investigation, our group introduced the TFAMoplex, a transfection agent based
on the mitochondrial transcription factor A (TFAM) protein, which complexes DNA into …

Microfluidic devices for continuous production of pDNA/cationic liposome complexes for gene delivery and vaccine therapy

TA Balbino, AR Azzoni, LG de La Torre - Colloids and Surfaces B …, 2013 - Elsevier
To evaluate the process parameters for the production of plasmid DNA/cationic liposome
(pDNA/CL) complexes in microfluidic systems, we studied two microfluidic devices: one with …

Building spatial synthetic biology with compartments, scaffolds, and communities

JK Polka, SG Hays, PA Silver - Cold Spring Harbor …, 2016 - cshperspectives.cshlp.org
Traditional views of synthetic biology often treat the cell as an unstructured container in
which biological reactions proceed uniformly. In reality, the organization of biological …

Development and characterization of a cationic lipid nanocarrier as non-viral vector for gene therapy

P Severino, M Szymanski, M Favaro, AR Azzoni… - European Journal of …, 2015 - Elsevier
The aim of the present work was to produce a cationic solid lipid nanoparticle (SLN) as non-
viral vector for protein delivery. Cationic SLN were produced by double emulsion method …

Microfluidic assembly of pDNA/Cationic liposome lipoplexes with high pDNA loading for gene delivery

TA Balbino, JM Serafin, AA Malfatti-Gasperini… - Langmuir, 2016 - ACS Publications
Microfluidics offers unique characteristics to control the mixing of liquids under laminar flow.
Its use for the assembly of lipoplexes represents an attractive alternative for the translation of …

Kinesin and dynein mechanics: measurement methods and research applications

Z Abraham, E Hawley, D Hayosh… - Journal of …, 2018 - asmedigitalcollection.asme.org
Motor proteins play critical roles in the normal function of cells and proper development of
organisms. Among motor proteins, failings in the normal function of two types of proteins …

Scalable production of highly concentrated chitosan/TPP nanoparticles in different pHs and evaluation of the in vitro transfection efficiency

CC Sipoli, N Santana, AAM Shimojo, A Azzoni… - Biochemical …, 2015 - Elsevier
Chitosan (CHI) pentasodium tripolyphosphate (TPP) nanoparticles have been studied
extensively for biomedical applications. However, these studies do not attempt to develop a …