Biologically active proteins are useful for studying the biological functions of genes and for
the development of therapeutic drugs and biomaterials in a biotechnology industry …

Recombinant proteins are often expressed in the form of insoluble inclusion bodies in
bacteria. To facilitate refolding of recombinant proteins obtained from inclusion bodies, 0.1 to …

Protein-protein interaction (PPI) is a hot topic in clinical research as protein networking has a
major impact in human disease. Such PPIs are potential drugs targets, leading to the need …

Arginine is one of the universal reagents that are effective in assisting refolding of
recombinant proteins from inclusion bodies. The mechanism of the effects of arginine on …

This paper overviews solution additives that affect protein stability and aggregation during
refolding, heating, and freezing processes. Solution additives are mainly grouped into two …

The gradual removal of the denaturing reagent guanidine HCl (GdnHCl) using stepwise
dialysis with the introduction of an oxidizing reagent and l-arginine resulted in the highly …

Abstract l‐Arginine (l‐Arg) has been widely used as an enhancer of protein renaturation.
The mechanism behind its action is still not fully understood. Using hen egg white lysozyme …

Abstract Purpose: Human interleukin-21 (IL-21) is a pleiotropic class I cytokine that activates
CD8+ T cells and natural killer cells. We report a phase 1 study of recombinant human IL-21 …

Intrinsically disordered, highly charged protein sequences act as entropic bristles (EBs),
which, when translationally fused to partner proteins, serve as effective solubilizers by …

L-Arginine is one of the most commonly used and most generally applicable suppressors of
protein aggregation. Its effect as enhancer of in vitro protein refolding was serendipitously …