In 1970, three laboratories independently made a discovery that, for aromatic fluorophores
embedded into different rigid and highly viscous media, the spectroscopic properties do not …

This chapter reviews basic concepts of nonlinear fluorescence upconversion, a technique
whose temporal resolution is essentially limited only by the pulse width of the ultrafast laser …

Photonics of biopolymers discusses the processes of energy transformation in photoexcited
proteins, nucleic acids, membranes and model systems. The author addresses, among other …

Design and characterization of a general-purpose spectrometer for recording time-resolved
emission spectra of typical fluorescent species is described. The system is based on a high …

Time-resolved Stokes-shift experiments measure the dynamics of biomolecules and of the
perturbed solvent near them on subnanosecond time scales, but molecular dynamics …

Tryptophan (Trp) fluorescence is potentially a powerful probe for studying the conformational
ensembles of proteins in solution, as it is highly sensitive to the local electrostatic …

This work reports an explanation for the unusual monoexponential fluorescence decay of 5-
fluorotryptophan (5FTrp) in single-Trp mutant proteins [Broos, J.; Maddalena, F.; Hesp, BHJ …

Fluorosubstituted tryptophans serve as valuable probes for fluorescence and nuclear
magnetic resonance (NMR) studies of proteins. Here, we describe an unusual …

Human γD-crystallin (HγD-Crys) is a two-domain, β-sheet eye lens protein found in the lens
nucleus. Its long-term solubility and stability are important to maintain lens transparency …

Excited-state lifetime and quantum yield of a fluorophore are determined by the radiative
and nonradiative decay rates. There is an extensive literature about factors affecting the …