Transport of membranes and proteins in eukaryotic cells is mediated by vesicular carriers.
Here we review the biogenesis and functions of COPI vesicles, carriers that operate in the …

A variety of secretory cargoes move through the Golgi, but the pathways and mechanisms of
this traffic are still being debated. Here, we evaluate the strengths and weaknesses of five …

Coatomer complex I (COPI) mediates retrograde vesicular trafficking from Golgi to the
endoplasmic reticulum (ER) and within Golgi compartments. Deficiency in subunit alpha …

Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has
established itself as a key technology to study protein-protein interactions in living cells. A …

ADP-ribosylation factor (ARF) GTPases are major regulators of cellular membrane
homeostasis. High sequence similarity and multiple, possibly redundant functions of the five …

COPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the
endoplasmic reticulum. The structures of membrane protein coats, including COPI, have …

The coat protein complex I (COPI) allows the precise sorting of lipids and proteins between
Golgi cisternae and retrieval from the Golgi to the ER. This essential role maintains the …

Since its first visualization in 1898, the Golgi has been a topic of intense morphological
research. A typical mammalian Golgi consists of a pile of stapled cisternae, the Golgi stack …

Protein traffic is necessary to maintain homeostasis in all eukaryotic organisms. All newly
synthesized secretory proteins destined to the secretory and endolysosmal systems are …

The Golgi serves as a hub for intracellular membrane traffic in the eukaryotic cell. Transport
within the early secretory pathway, that is within the Golgi and from the Golgi to the …