How bacteria grow and divide while retaining a defined shape is a fundamental question in
microbiology, but technological advances are now driving a new understanding of how the …

Genetic interactions identify combinations of genetic variants that im**e on phenotype.
With whole-genome sequence information available for thousands of individuals within a …

A systems-level understanding of Gram-positive bacteria is important from both an
environmental and health perspective and is most easily obtained when high-quality …

Bacterial CRISPR-Cas systems utilize sequence-specific RNA-guided nucleases to defend
against bacteriophage infection. As a countermeasure, numerous phages are known that …

Highlights•Combinations of antibiotics lead to synergistic and antagonistic drug
interactions.•The underlying mechanisms of drug interactions can be elucidated using novel …

The explosion of sequence information in bacteria makes develo** high-throughput, cost-
effective approaches to matching genes with phenotypes imperative. Using E. coli as proof …

Biological pathways are structured in complex networks of interacting genes. Solving the
architecture of such networks may provide valuable information, such as how …

Genetic interactions identify functional connections between genes and pathways,
establishing gene functions or druggable targets. Here we use CRISPRi–TnSeq, CRISPRi …

Protein and genetic interaction maps can reveal the overall physical and functional
landscape of a biological system. To date, these interaction maps have typically been …

Synthetic lethality occurs when the inhibition of two genes is lethal while the inhibition of
each single gene is not. It can be harnessed to selectively treat cancer by identifying inactive …