The budding yeast Saccharomyces cerevisiae has been the principal organism used in
experiments to examine genetic recombination in eukaryotes. Studies over the past decade …

Cell cycle checkpoints are regulatory pathways that control the order and timing of cell cycle
transitions and ensure that critical events such as DNA replication and chromosome …

The two-hybrid system is a powerful technique for detecting protein-protein interactions that
utilizes the well-developed molecular genetics of the yeast Saccharomyces cerevisiae …

Background: The response of eukaryotic cells to double-strand breaks in genomic DNA
includes the sequestration of many factors into nuclear foci. Recently it has been reported …

Chk1, an evolutionarily conserved protein kinase, has been implicated in cell cycle
checkpoint control in lower eukaryotes. By gene disruption, we show that CHK1 deficiency …

In response to DNA damage and replication blocks, cells prevent cell cycle progression
through the control of critical cell cycle regulators. We identified Chk2, the mammalian …

At close hand to one's genomic material are the histones that make up the nucleosome.
Standing guard, one variant stays hidden doubling as one of the core histones. But, thanks …

Cells are constantly under threat from the cytotoxic and mutagenic effects of DNA damaging
agents. These agents can either be exogenous or formed within cells. Environmental DNA …

From bacteria to mammalian cells, damaged DNA is sensed and targeted by DNA repair
pathways. In eukaryotes, kinases play a central role in coordinating the DNA damage …

p53 binding protein 1 (53BP1), a protein proposed to function as a transcriptional coactivator
of the p53 tumor suppressor, has BRCT domains with high homology to the Saccharomyces …