According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

Protein glycosylation is an important post-translational modification. It is a feature that
enhances the functional diversity of proteins and influences their biological activity. A wide …

Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem
mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene anions transfer an …

Mass spectrometry is the main analytical technique currently used to address the challenges
of glycomics as it offers unrivalled levels of sensitivity and the ability to handle complex …

The top-down approach to proteomics offers compelling advantages due to the potential to
provide complete characterization of protein sequence and post-translational modifications …

Mass spectrometry (MS)–based high-throughput proteomics is the core technique for large-
scale protein characterization. Due to the extreme complexity of proteomes, sophisticated …

This tutorial presents the most common ion activation techniques employed in tandem mass
spectrometry. In‐source fragmentation and metastable ion decompositions, as well as the …

Highlights•Glycosylation of antibodies has a range of effects on their binding and effector
functions.•Regulatory agencies require stringent glycan analysis to ensure safety and …

Over the past decade, mass spectrometry has been revolutionized by access to instruments
of increasingly high mass-resolving power. For small molecules up to∼ 400 Da (eg, drugs …

Replicate mass spectrometry (MS) measurements and the use of multiple analytical
methods can greatly expand the comprehensiveness of shotgun proteomic profiling of …