The recent advances in DNA sequencing technology are enabling a rapid increase in the
number of genomes being sequenced. However, many fundamental questions in genome …

Hundreds of vertebrate genomes have been sequenced and assembled to date. However,
most sequencing projects have ignored the sex chromosomes unique to the heterogametic …

Apes possess two sex chromosomes—the male-specific Y chromosome and the X
chromosome, which is present in both males and females. The Y chromosome is crucial for …

The decrease in sequencing cost and increased sophistication of assembly algorithms for
short-read platforms has resulted in a sharp increase in the number of species with genome …

Since its initial release in 2001, the human reference genome has undergone continuous
improvement in quality, and the recently released telomere-to-telomere (T2T) version—T2T …

Background Heteromorphic sex chromosomes have evolved repeatedly across diverse
species. Suppression of recombination between X and Y chromosomes leads to …

We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-
resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb …

Heterochromatic repeat-rich regions are often missing from even the best genome
assemblies. Chang and Larracuente designed a de novo assembly strategy to improve the …

Background Sex determination mechanisms in teleost fish broadly differ from mammals and
birds, with sex chromosomes that are far less differentiated and recombination often …

Teleosts are important models to study sex chromosomes and sex-determining (SD) genes
because they present a variety of sex determination systems. Here, we used Nanopore and …