Measurement of the electrostatic interactions that give rise to biological functions has been a
longstanding challenge in biophysics. Advances in spectroscopic techniques over the past …

Green fluorescent proteins (GFPs) have become indispensable imaging and optogenetic
tools. Their absorption and emission properties can be optimized for specific applications …

High-resolution synchrotron and neutron single-crystal diffraction data of 18-crown-
6/(pentakis) urea measured at 30 K are combined, with the aim of better appreciating the …

This article summarizes a series of seventeen publications by the authors devoted to
molecular dynamics modeling of various indicator dyes (molecular probes) enclosed in …

The role cysteine residues play in proteins is mediated by their protonation state, whereby
the thiolate form of the side chain is highly reactive while the thiol form is more inert …

Fluorescent proteins (FPs) have had an enormous impact on molecular and cellular biology
and are employed in a wide range of studies of molecular structure and dynamics. Yet, only …

Mutations of human oncoprotein p21H-Ras (hereafter “Ras”) at glutamine 61 are known to
slow the rate of guanosine triphosphate (GTP) hydrolysis and transform healthy cells into …

Vibrational spectroscopy is a powerful tool for characterizing the complex noncovalent
interactions that arise in biological systems. The nitrile stretching frequency has proven to be …

Thioflavin T (ThT) is a typical fluorescent marker for detecting the formation of amyloid fibrils,
because its fluorescence intensity increases by more than 2 orders of magnitude upon …

A vibration's transition frequency is partly determined by the first-order Stark effect, which
accounts for the electric field experienced by the mode. Using ultrafast infrared pump–probe …