Microbioreactor systems for accelerated bioprocess development

J Hemmerich, S Noack, W Wiechert… - Biotechnology …, 2018 - Wiley Online Library
In recent years, microbioreactor (MBR) systems have evolved towards versatile bioprocess
engineering tools. They provide a unique solution to combine higher experimental …

[HTML][HTML] Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNA

B Koopal, A Potocnik, SK Mutte, C Aparicio-Maldonado… - Cell, 2022 - cell.com
Argonaute proteins use single-stranded RNA or DNA guides to target complementary
nucleic acids. This allows eukaryotic Argonaute proteins to mediate RNA interference and …

General calibration of microbial growth in microplate readers

K Stevenson, AF McVey, IBN Clark, PS Swain… - Scientific reports, 2016 - nature.com
Optical density (OD) measurements of microbial growth are one of the most common
techniques used in microbiology, with applications ranging from studies of antibiotic efficacy …

Robust estimation of bacterial cell count from optical density

J Beal, NG Farny, T Haddock-Angelli… - Communications …, 2020 - nature.com
Optical density (OD) is widely used to estimate the density of cells in liquid culture, but
cannot be compared between instruments without a standardized calibration protocol and is …

Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria

RC Hurt, MT Buss, M Duan, K Wong, MY You… - Nature …, 2023 - nature.com
Ultrasound allows imaging at a much greater depth than optical methods, but existing
genetically encoded acoustic reporters for in vivo cellular imaging have been limited by poor …

Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology

N Radde, GA Mortensen, D Bhat, S Shah… - Nature …, 2024 - nature.com
Engineered DNA will slow the growth of a host cell if it redirects limiting resources or
otherwise interferes with homeostasis. Escape mutants that alleviate this burden can rapidly …

Effects of activated carbon on liquefaction resistance of calcareous sand treated with microbially induced calcium carbonate precipitation

Y Shan, J Zhao, H Tong, J Yuan, D Lei, Y Li - Soil Dynamics and …, 2022 - Elsevier
Microbially induced calcium carbonate precipitation (MICP) is a new, natural,
environmentally friendly treatment method for soil foundations, which can improve the …

Droplet printing reveals the importance of micron-scale structure for bacterial ecology

R Krishna Kumar, TA Meiller-Legrand… - Nature …, 2021 - nature.com
Bacteria often live in diverse communities where the spatial arrangement of strains and
species is considered critical for their ecology. However, a test of this hypothesis requires …

In situ characterisation and manipulation of biological systems with Chi. Bio

H Steel, R Habgood, CL Kelly… - PLoS biology, 2020 - journals.plos.org
The precision and repeatability of in vivo biological studies is predicated upon methods for
isolating a targeted subsystem from external sources of noise and variability. However, in …

Pulsatile inputs achieve tunable attenuation of gene expression variability and graded multi-gene regulation

D Benzinger, M Khammash - Nature communications, 2018 - nature.com
Many natural transcription factors are regulated in a pulsatile fashion, but it remains
unknown whether synthetic gene expression systems can benefit from such dynamic …