Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

In this Review article, we systematically summarize the design and applications of various
kinds of long-lived emissive probes for bioimaging and biosensing via time-resolved …

Fluorescence lifetime imaging microscopy (FLIM) is used in diverse disciplines, including
biology, chemistry and biophysics, but its use has been limited by the complexity of the data …

When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Changing the data representation from the classical time delay histogram to the phasor
representation provides a global view of the fluorescence decay at each pixel of an image …

Raman spectroscopy is an important and powerful technique for analyzing the chemical
composition of biological or nonbiological samples in many fields. A serious challenge …

We describe a label-free imaging method to monitor stem-cell metabolism that discriminates
different states of stem cells as they differentiate in living tissues. In this method we use …

The phasor approach to fluorescence lifetime imaging has become a common method to
analyze complicated fluorescence signals from biological samples. The appeal of the …

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

The Kohlrausch (stretched exponential) decay law is analyzed in detail. Analytical and
approximate forms of the distribution of rate constants and related functions are obtained for …