Structures from intact myofibrils reveal mechanism of thin filament regulation through nebulin

Z Wang, M Grange, S Pospich, T Wagner, AL Kho… - Science, 2022 - science.org
In skeletal muscle, nebulin stabilizes and regulates the length of thin filaments, but the
underlying mechanism remains nebulous. In this work, we used cryo–electron tomography …

A new generation of crystallographic validation tools for the protein data bank

RJ Read, PD Adams, WB Arendall, AT Brunger… - Structure, 2011 - cell.com
This report presents the conclusions of the X-ray Validation Task Force of the worldwide
Protein Data Bank (PDB). The PDB has expanded massively since current criteria for …

The SARS-CoV-2 conserved macrodomain is a mono-ADP-ribosylhydrolase

YMO Alhammad, MM Kashipathy, A Roy… - Journal of …, 2021 - Am Soc Microbiol
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other SARS-related
CoVs encode 3 tandem macrodomains within nonstructural protein 3 (nsp3). The first …

Bump-and-hole engineering identifies specific substrates of glycosyltransferases in living cells

B Schumann, SA Malaker, SP Wisnovsky, MF Debets… - Molecular cell, 2020 - cell.com
Studying posttranslational modifications classically relies on experimental strategies that
oversimplify the complex biosynthetic machineries of living cells. Protein glycosylation …

Structure and function of the PiuA and PirA siderophore-drug receptors from Pseudomonas aeruginosa and Acinetobacter baumannii

L Moynié, A Luscher, D Rolo, D Pletzer… - Antimicrobial agents …, 2017 - Am Soc Microbiol
The outer membrane of Gram-negative bacteria presents an efficient barrier to the
permeation of antimicrobial molecules. One strategy pursued to circumvent this obstacle is …

Active-site conformational fluctuations promote the enzymatic activity of NDM-1

H Zhang, G Ma, Y Zhu, L Zeng, A Ahmad… - Antimicrobial agents …, 2018 - Am Soc Microbiol
ABSTRACT β-Lactam antibiotics are the mainstay for the treatment of bacterial infections.
However, elevated resistance to these antibiotics mediated by metallo-β-lactamases (MBLs) …

Structural analysis of cholesterol binding and sterol selectivity by ABCG5/G8

D Farhat, F Rezaei, M Ristovski, Y Yang… - Journal of molecular …, 2022 - Elsevier
The ATP-binding cassette (ABC) sterol transporters are responsible for maintaining
cholesterol homeostasis in mammals by participating in reverse cholesterol transport (RCT) …

Structural/mechanistic insights into the efficacy of nonclassical β‐lactamase inhibitors against extensively drug resistant Stenotrophomonas maltophilia clinical …

K Calvopiña, P Hinchliffe, J Brem… - Molecular …, 2017 - Wiley Online Library
Clavulanic acid and avibactam are clinically deployed serine β‐lactamase inhibitors,
important as a defence against antibacterial resistance. Bicyclic boronates are recently …

Alternative Recognition of the Conserved Stem Epitope in Influenza A Virus Hemagglutinin by a VH3-30-Encoded Heterosubtypic Antibody

A Wyrzucki, C Dreyfus, I Kohler, M Steck… - Journal of …, 2014 - Am Soc Microbiol
ABSTRACT A human monoclonal heterosubtypic antibody, MAb 3.1, with its heavy chain
encoded by VH3-30, was isolated using phage display with immobilized hemagglutinin (HA) …

Structural and molecular dynamics studies of a C1‐oxidizing lytic polysaccharide monooxygenase from Heterobasidion irregulare reveal amino acids important for …

B Liu, AA Kognole, M Wu, B Westereng… - The FEBS …, 2018 - Wiley Online Library
Lytic polysaccharide monooxygenases (LPMO s) are a group of recently discovered
enzymes that play important roles in the decomposition of recalcitrant polysaccharides …