All organisms produce specialized organic molecules, ranging from small volatile chemicals
to large gene-encoded peptides, that have evolved to provide them with diverse cellular and …

The cryptic secondary metabolite biosynthetic gene clusters (BGCs) far outnumber currently
known secondary metabolites. Heterologous production of secondary metabolite BGCs in …

Methods for in vitro DNA cleavage and molecular cloning remain unable to precisely cleave
DNA directly adjacent to bases of interest. Restriction enzymes (REs) must bind specific …

Direct cloning of biosynthetic gene clusters (BGCs) from microbial genomes facilitates
natural product-based drug discovery. Here, by combining Cas12a and the advanced …

The era of inexpensive genome sequencing and improved bioinformatics tools has
reenergized the study of natural products, including the ribosomally synthesized and post …

Natural products have long served as critical raw materials in chemical and pharmaceutical
manufacturing, primarily which can provide superior scaffolds or intermediates for drug …

CRISPR-associated Cas12 proteins are a highly variable collection of nucleic acid-targeting
proteins. All Cas12 variants use RNA guides and a single nuclease domain to target …

Recent efforts in genome mining of ribosomally synthesized and post-translationally
modified peptides (RiPPs) have expanded the diversity of post-translational modification …

The rise of antibiotic-resistant bacteria represents a major threat to global health, creating an
urgent need to discover new antibiotics. Natural products derived from the genus …

Bacterial genomes contain large reservoirs of biosynthetic gene clusters (BGCs) that are
predicted to encode unexplored natural products. Heterologous expression of previously …