Prokaryotic Argonautes for in vivo biotechnology and molecular diagnostics

BA Graver, N Chakravarty, KV Solomon - Trends in biotechnology, 2024‏ - cell.com
Prokaryotic Argonautes (pAgos) are an emerging class of programmable endonucleases
that are believed to be more flexible than existing CRISPR–Cas systems and have …

Improvements of nuclease and nickase gene modification techniques for the treatment of genetic diseases

Y Lu, C Happi Mbakam, B Song, E Bendavid… - Frontiers in Genome …, 2022‏ - frontiersin.org
Advancements in genome editing make possible to exploit the functions of enzymes for
efficient DNA modifications with tremendous potential to treat human genetic diseases …

Peptide nucleic acid-assisted generation of targeted double-stranded DNA breaks with T7 endonuclease I

R Aman, MM Syed, A Saleh, F Melliti… - Nucleic Acids …, 2024‏ - academic.oup.com
Gene-editing technologies have revolutionized biotechnology, but current gene editors
suffer from several limitations. Here, we harnessed the power of gamma-modified peptide …

A photoresponsive homing endonuclease for programmed DNA cleavage

LA Johnson, RJ Mart, RK Allemann - ACS Synthetic Biology, 2023‏ - ACS Publications
Homing endonucleases are used in a wide range of biotechnological applications including
gene editing, in gene drive systems, and for the modification of DNA structures, arrays, and …

Positive Selection Screens for Programmable Endonuclease Activity Using I-SceI

MA Mechikoff, KZ Lee, KV Solomon - Synthetic Biology: Methods and …, 2024‏ - Springer
Positive selection screens are high-throughput assays to characterize novel enzymes from
environmental samples and enrich for more powerful variants from libraries in applications …

Preliminary Exploration of a DNA Editing Strategy to Treat Cystic Fibrosis using Homology-Independent Targeted Integration and Receptor-Targeted Nanoparticles

C Graham - 2024‏ - discovery.ucl.ac.uk
Cystic fibrosis (CF) is a common life-limiting disorder. Dysfunctional cystic fibrosis
transmembrane conductance regulator (CFTR) protein hinders mucociliary clearance in the …

Programmable Control of Protein Activity Via Formation of Biomolecular Condensates in Bacteria

MK Parasa - 2023‏ - hammer.purdue.edu
Biomolecular condensates or membrane-less organelles are phase separated proteins
and/or other biomolecules that are ubiquitous in eukaryotic cells. While these condensates …