Discerning conformational dynamics and binding kinetics of GPCRs by 19F NMR
RS Prosser, NA Alonzi - Current Opinion in Pharmacology, 2023 - Elsevier
Abstract 19 F NMR provides a way of monitoring conformational dynamics of G-protein
coupled receptors (GPCRs) from the perspective of an ensemble. While X-ray …
coupled receptors (GPCRs) from the perspective of an ensemble. While X-ray …
Determination of the pKa and Concentration of NMR-Invisible Molecules and Sites Using NMR Spectroscopy
H Hussain, YZ Khimyak, M Wallace - Analytical Chemistry, 2024 - ACS Publications
NMR spectroscopy is a very powerful tool for measuring the dissociation constants (p K a) of
molecules, requiring smaller quantities of samples of lower purity relative to potentiometric …
molecules, requiring smaller quantities of samples of lower purity relative to potentiometric …
[HTML][HTML] Localized apelin-17 analogue-bicelle interactions as a facilitator of membrane-catalyzed receptor recognition and binding
The apelinergic system encompasses two peptide ligand families, apelin and apela, along
with the apelin receptor (AR or APJ), a class A G-protein-coupled receptor. This system has …
with the apelin receptor (AR or APJ), a class A G-protein-coupled receptor. This system has …
Nucleotide Binding and Active Site Gate Dynamics for the Hsp90 Chaperone ATPase Domain from Benchtop and High Field 19F NMR Spectroscopy
Protein turnover in cells is regulated by the ATP dependent activity of the Hsp90 chaperone.
In concert with accessory proteins, ATP hydrolysis drives the obligate Hsp90 dimer through …
In concert with accessory proteins, ATP hydrolysis drives the obligate Hsp90 dimer through …
Structure and Function of Membrane Proteins
L Fliegel - International Journal of Molecular Sciences, 2023 - mdpi.com
While we have a great deal of information on the human genome, in many cases we still
know little about the structure's function, the regulation of membrane proteins and how they …
know little about the structure's function, the regulation of membrane proteins and how they …
On-cell nuclear magnetic resonance spectroscopy to probe cell surface interactions
Nuclear magnetic resonance (NMR) spectroscopy allows the determination of atomic-level
information on intermolecular interactions, molecular structure, and molecular dynamics in …
information on intermolecular interactions, molecular structure, and molecular dynamics in …
Exploring potential new receptors for ELABELA in human embryonic stem cells by Crispr/Cas9-mediated gene editing system
Z Yue, C CHENG, E ZHENG, M Zhuo… - Journal of Shanghai …, 2022 - xuebao.shsmu.edu.cn
Objective To verify the existence of potential new receptor of ELABELA (ELA) in human
embryonic stem cells (hESC) by Crispr/Cas9-mediated gene editing system. Methods The …
embryonic stem cells (hESC) by Crispr/Cas9-mediated gene editing system. Methods The …
利用 Crispr/Cas9 基因编辑系统在人胚胎干细胞中探索 ELABELA 的潜在新受体
周月， 程晨， 郑恩霖， 孟卓， 王鉴… - 上海交通大学学报 …, 2022 - xuebao.shsmu.edu.cn
目的· 利用Crispr/Cas9 基因编辑技术在人胚胎干细胞(human embryonic stem cell, hESC)
中探索小分子多肽ELABELA (ELA) 是否存在潜在的新受体. 方法· 收集干细胞向心肌细胞定向 …
中探索小分子多肽ELABELA (ELA) 是否存在潜在的新受体. 方法· 收集干细胞向心肌细胞定向 …