Identifying biases and their potential solutions in human microbiome studies

JT Nearing, AM Comeau, MGI Langille - Microbiome, 2021 - Springer
Advances in DNA sequencing technology have vastly improved the ability of researchers to
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …

Reintroducing mothur: 10 years later

PD Schloss - Applied and environmental microbiology, 2020 - journals.asm.org
More than 10 years ago, we published the paper describing the mothur software package in
Applied and Environmental Microbiology. Our goal was to create a comprehensive package …

Primer and platform effects on 16S rRNA tag sequencing

J Tremblay, K Singh, A Fern, ES Kirton, S He… - Frontiers in …, 2015 - frontiersin.org
Sequencing of 16S rRNA gene tags is a popular method for profiling and comparing
microbial communities. The protocols and methods used, however, vary considerably with …

Tag jumps illuminated–reducing sequence‐to‐sample misidentifications in metabarcoding studies

IB Schnell, K Bohmann… - Molecular ecology …, 2015 - Wiley Online Library
Metabarcoding of environmental samples on second‐generation sequencing platforms has
rapidly become a valuable tool for ecological studies. A fundamental assumption of this …

Who is eating what: diet assessment using next generation sequencing

F Pompanon, BE Deagle, WOC Symondson… - Molecular …, 2012 - Wiley Online Library
The analysis of food webs and their dynamics facilitates understanding of the mechanistic
processes behind community ecology and ecosystem functions. Having accurate techniques …

Next‐generation sequencing technologies for environmental DNA research

S Shokralla, JL Spall, JF Gibson… - Molecular …, 2012 - Wiley Online Library
Since 2005, advances in next‐generation sequencing technologies have revolutionized
biological science. The analysis of environmental DNA through the use of specific gene …

DNA metabarcoding multiplexing and validation of data accuracy for diet assessment: application to omnivorous diet

M De Barba, C Miquel, F Boyer… - Molecular ecology …, 2014 - Wiley Online Library
Ecological understanding of the role of consumer–resource interactions in natural food webs
is limited by the difficulty of accurately and efficiently determining the complex variety of food …

Strategies for sample labelling and library preparation in DNA metabarcoding studies

K Bohmann, V Elbrecht, C Carøe, I Bista… - Molecular ecology …, 2022 - Wiley Online Library
Metabarcoding of DNA extracted from environmental or bulk specimen samples is
increasingly used to profile biota in basic and applied biodiversity research because of its …

Microbial populations responsible for specific soil suppressiveness to plant pathogens

DM Weller, JM Raaijmakers… - Annual review of …, 2002 - annualreviews.org
▪ Abstract Agricultural soils suppressive to soilborne plant pathogens occur worldwide, and
for several of these soils the biological basis of suppressiveness has been described. Two …

Soil microbial community responses to multiple experimental climate change drivers

HF Castro, AT Classen, EE Austin… - Applied and …, 2010 - journals.asm.org
Researchers agree that climate change factors such as rising atmospheric [CO2] and
warming will likely interact to modify ecosystem properties and processes. However, the …