The advent of next-generation sequencing (NGS) technologies has expanded our ability to
detect and analyze microbial genomes and has yielded novel molecular approaches for …

Whole genome sequencing (WGS) provides the highest resolution for genome-based
species identification and can provide insight into the antimicrobial resistance and virulence …

Background Shotgun metagenomics has been used clinically for diagnosing infectious
diseases. However, most technical assessments have been limited to individual sets of …

Emerging and endemic zoonotic diseases continue to threaten human and animal health,
our social fabric, and the global economy. Zoonoses frequently emerge from congregate …

Background As most viruses remain uncultivated, metagenomics is currently the main
method for virus discovery. Detecting viruses in metagenomic data is not trivial. In the past …

Introduction Meningoencephalitis patients are often severely impaired and benefit from early
etiological diagnosis, though many cases remain without identified cause. Metagenomics as …

Background Laboratory-developed metagenomic next-generation sequencing (mNGS)
assays are increasingly being used for the diagnosis of infectious disease. To ensure …

Viruses are the main agents causing emerging and re-emerging infectious diseases. It is
therefore important to screen for and detect them and uncover the evolutionary processes …

The field of biosecurity has greatly benefited from the widespread adoption of high-
throughput sequencing technologies, for its ability to deeply query plant and animal samples …

Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of
detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory …