Strategies for genetic manipulation of adult stem cell-derived organoids
C Menche, HF Farin - Experimental & Molecular Medicine, 2021 - nature.com
Organoid technology allows the expansion of primary epithelial cells from normal and
diseased tissues, providing a unique model for human (patho) biology. In a three …
diseased tissues, providing a unique model for human (patho) biology. In a three …
Enrichment strategies to enhance genome editing
NS Mikkelsen, RO Bak - Journal of Biomedical Science, 2023 - Springer
Genome editing technologies hold great promise for numerous applications including the
understanding of cellular and disease mechanisms and the development of gene and …
understanding of cellular and disease mechanisms and the development of gene and …
[HTML][HTML] Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR …
C Vogeley, NC Sondermann, S Woeste… - Environment …, 2022 - Elsevier
Polycyclic aromatic hydrocarbons (PAHs), dioxin-like compounds (DLCs) and structurally-
related environmental pollutants may contribute to the pathogenesis of various diseases and …
related environmental pollutants may contribute to the pathogenesis of various diseases and …
Assessing the NLRP3 inflammasome activating potential of a large panel of micro-and nanoplastics in THP-1 cells
M Busch, G Bredeck, F Waag, K Rahimi… - Biomolecules, 2022 - mdpi.com
Due to the ubiquity of environmental micro-and nanoplastics (MNPs), inhalation and
ingestion by humans is very likely, but human health effects remain largely unknown. The …
ingestion by humans is very likely, but human health effects remain largely unknown. The …
[HTML][HTML] Inhalable Saharan dust induces oxidative stress, NLRP3 inflammasome activation, and inflammatory cytokine release
Desert dust is increasingly recognized as a major air pollutant affecting respiratory health.
Since desert dust exposure cannot be regulated, the hazardousness of its components must …
Since desert dust exposure cannot be regulated, the hazardousness of its components must …
Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture model
Background Epidemiological studies have related desert dust events to increased
respiratory morbidity and mortality. Although the Sahara is the largest source of desert dust …
respiratory morbidity and mortality. Although the Sahara is the largest source of desert dust …
Mitochondrial DNA integrity and metabolome profile are preserved in the human induced pluripotent stem cell reference line KOLF2. 1J
Quality control of human induced pluripotent stem cells (iPSCs) is critical to ensure
reproducibility of research. Recently, KOLF2. 1J was characterized and published as a male …
reproducibility of research. Recently, KOLF2. 1J was characterized and published as a male …
Making gene editing accessible in resource limited environments: recommendations to guide a first-time user
S Goolab, J Scholefield - Frontiers in Genome Editing, 2024 - frontiersin.org
The designer nuclease, CRISPR-Cas9 system has advanced the field of genome
engineering owing to its programmability and ease of use. The application of these …
engineering owing to its programmability and ease of use. The application of these …
Investigating the role of the NLRP3 inflammasome pathway in acute intestinal inflammation: Use of THP-1 knockout cell lines in an advanced triple culture model
The NLRP3 inflammasome plays an important role in intestinal homeostasis as well as
inflammation. However, in vivo studies investigating the role of the NLRP3 inflammasome in …
inflammation. However, in vivo studies investigating the role of the NLRP3 inflammasome in …
Reassessment of marker genes in human induced pluripotent stem cells for enhanced quality control
Human induced pluripotent stem cells (iPSCs) have great potential in research, but
pluripotency testing faces challenges due to non-standardized methods and ambiguous …
pluripotency testing faces challenges due to non-standardized methods and ambiguous …