Signaling ammonium across membranes through an ammonium sensor histidine kinase

T Pflüger, CF Hernández, P Lewe, F Frank… - Nature …, 2018 - nature.com
Sensing and uptake of external ammonium is essential for anaerobic ammonium-oxidizing
(anammox) bacteria, and is typically the domain of the ubiquitous Amt/Rh ammonium …

Direct phasing at low resolution of a protein copurified with human paraoxonase (PON1)

A Fokine, R Morales, C Contreras-Martel… - … Section D: Biological …, 2003 - journals.iucr.org
In this paper, the low-resolution structure of a previously unknown protein copurified with
human paraoxonase (PON1) is reported. The structure of this protein was very difficult to …

Macromolecular envelope determination and envelope-based phasing

Q Hao - Acta Crystallographica Section D: Biological …, 2006 - journals.iucr.org
Small-angle X-ray scattering (SAXS) or electron-microscopy (EM) data have proven to be
very useful in providing low-resolution structural details of proteins and other …

Connectivity-based ab initio phasing: from low resolution to a secondary structure

N Lunina, V Lunin, A Urzhumtsev - Acta Crystallographica Section …, 2003 - journals.iucr.org
The connectivity-based phasing method currently allows ab initio determination of phases
for several hundred reflections. In the case of large macromolecular crystals, these …

Molecular envelopes derived from protein powder diffraction data

JP Wright, C Besnard, I Margiolaki… - Journal of Applied …, 2008 - journals.iucr.org
The preparation of single crystals suitable for X-ray analysis is frequently the most difficult
step in structural studies of proteins. With the aid of two examples, it is shown that de novo …

Low-resolution structure determination of Na+-translocating NADH: ubiquinone oxidoreductase from Vibrio cholerae by ab initio phasing and electron microscopy

VY Lunin, NL Lunina, MS Casutt, K Knoops… - … Section D: Biological …, 2012 - scripts.iucr.org
A low-resolution structure of the Na+-translocating NADH: ubiquinone oxidoreductase from
the human pathogen Vibrio cholerae was determined by ab initio phasing and …

Combining solution wide-angle X-ray scattering and crystallography: determination of molecular envelope and heavy-atom sites

X Hong, Q Hao - Journal of Applied Crystallography, 2009 - journals.iucr.org
Solving the phase problem remains central to crystallographic structure determination. A six-
dimensional search method of molecular replacement (FSEARCH) can be used to locate a …

PAN-modular structure of microneme protein SML-2 from the parasite Sarcocystis muris at 1.95 Å resolution and its complex with 1-thio-β-D-galactose

JJ Müller, MS Weiss, U Heinemann - … Crystallographica Section D …, 2011 - journals.iucr.org
The microneme protein SML-2 is a member of a small family of galactose-specific lectins that
play a role during host-cell invasion by the apicomplexan parasite Sarcocystis muris. The …

Ab initio phasing of low-resolution Fourier syntheses

VY Lunin, AG Urzhumtsev, A Podjarny - 2012 - Wiley Online Library
Low-resolution phasing addresses the cases where experimental X-ray diffraction intensities
are only available to a low-resolution limit, or when the standard phasing methods to solve …

Low-resolution ab initio phasing of Sarcocystis muris lectin SML-2

JJ Müller, NL Lunina, A Urzhumtsev… - … Section D: Biological …, 2006 - journals.iucr.org
Structural analysis of the lectin SML-2 faced difficulties when applying standard
crystallographic phasing methods. The connectivity-based ab initio phasing method allowed …