Protein purification and analysis: next generation Western blotting techniques

M Mishra, S Tiwari, AV Gomes - Expert review of proteomics, 2017 - Taylor & Francis
Introduction: Western blotting is one of the most commonly used techniques in molecular
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …

Western blotting

BT Kurien, RH Scofield - Methods, 2006 - Elsevier
Western blotting (protein blotting or immunoblotting) is a powerful and important procedure
for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low …

Western blotting: an introduction

BT Kurien, RH Scofield - Western blotting: Methods and protocols, 2015 - Springer
Western blotting is an important procedure for the immunodetection of proteins, particularly
proteins that are of low abundance. This process involves the transfer of protein patterns …

The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly

GJ Pazour, BL Dickert, GB Witman - The Journal of cell biology, 1999 - rupress.org
Dyneins are microtubule-based molecular motors involved in many different types of cell
movement. Most dynein heavy chains (DHCs) clearly group into cytoplasmic or axonemal …

[HTML][HTML] Molecular cloning of cDNA encoding human and rabbit forms of the Ca2+ release channel (ryanodine receptor) of skeletal muscle sarcoplasmic reticulum.

F Zorzato, J Fujii, K Otsu, M Phillips, NM Green… - Journal of Biological …, 1990 - Elsevier
We have cloned cDNAs encoding the rabbit and human forms of the Ca2+ release channel
of sarcoplasmic reticulum. The human cDNA encodes a protein of 5032 amino acids, with a …

Protein blotting: a review

BT Kurien, RH Scofield - Journal of immunological methods, 2003 - Elsevier
The utility of the high resolving power of gel electrophoresis was limited in purpose, owing to
the fact that the separated proteins in the gel matrix were difficult to access with molecular …

Induction of calcium currents by the expression of the α1-subunit of the dihydropyridine receptor from skeletal muscle

E Perez–Reyes, HS Kim, AE Lacerda, W Horne, X Wei… - Nature, 1989 - nature.com
THE dihydropyridine (DHP) receptor purified from skeletal muscle comprises five protein
subunits (α 1, α 2, β, γ and δ) and produces Ca2+ currents that are blocked by DHPs1 …

Multiplex Western blotting system for the analysis of muscular dystrophy proteins

LVB Anderson, K Davison - The American Journal of Pathology, 1999 - Elsevier
A multiplex system of Western blotting is presented in which most of the current muscular
dystrophy proteins can be analyzed simultaneously on one pair of blots. This represents a …

Spatio-temporal expression patterns of steroidogenic acute regulatory protein (StAR) during follicular development in the rat ovary

T Ronen-Fuhrmann, R Timberg, SR King… - …, 1998 - academic.oup.com
The steroidogenic acute regulatory protein (StAR) is a vital mitochondrial protein that is
indispensable for the synthesis of steroid hormones in the steroidogenic cells of the adrenal …

Abnormal protein turnover and anabolic resistance to exercise in sarcopenic obesity

MI Nilsson, JP Dobson, NP Greene… - The FASEB …, 2013 - Wiley Online Library
Obesity may impair protein synthesis rates and cause anabolic resistance to growth factors,
hormones, and exercise, ultimately affecting skeletal muscle mass and function. To better …