The technological landscape and applications of single-cell multi-omics

A Baysoy, Z Bai, R Satija, R Fan - Nature Reviews Molecular Cell …, 2023 - nature.com
Single-cell multi-omics technologies and methods characterize cell states and activities by
simultaneously integrating various single-modality omics methods that profile the …

The expanding vistas of spatial transcriptomics

L Tian, F Chen, EZ Macosko - Nature Biotechnology, 2023 - nature.com
The formation and maintenance of tissue integrity requires complex, coordinated activities
by thousands of genes and their encoded products. Until recently, transcript levels could …

An integrated cell atlas of the lung in health and disease

L Sikkema, C Ramírez-Suástegui, DC Strobl… - Nature medicine, 2023 - nature.com
Single-cell technologies have transformed our understanding of human tissues. Yet, studies
typically capture only a limited number of donors and disagree on cell type definitions …

Cross-tissue immune cell analysis reveals tissue-specific features in humans

C Domínguez Conde, C Xu, LB Jarvis, DB Rainbow… - Science, 2022 - science.org
Despite their crucial role in health and disease, our knowledge of immune cells within
human tissues remains limited. We surveyed the immune compartment of 16 tissues from 12 …

Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts infectivity and fusogenicity

B Meng, A Abdullahi, IATM Ferreira, N Goonawardane… - Nature, 2022 - nature.com
Abstract The SARS-CoV-2 Omicron BA. 1 variant emerged in 2021 and has multiple
mutations in its spike protein. Here we show that the spike protein of Omicron has a higher …

Map** spatially resolved transcriptomes in human and mouse pulmonary fibrosis

L Franzén, M Olsson Lindvall, M Hühn, V Ptasinski… - Nature Genetics, 2024 - nature.com
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with poor prognosis and
limited treatment options. Efforts to identify effective treatments are thwarted by limited …

Single-cell atlases: shared and tissue-specific cell types across human organs

R Elmentaite, C Domínguez Conde, L Yang… - Nature Reviews …, 2022 - nature.com
The development of single-cell and spatial transcriptomics methods was instrumental in the
conception of the Human Cell Atlas initiative, which aims to generate an integrated map of …

A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates

P He, K Lim, D Sun, JP Pett, Q Jeng, K Polanski… - Cell, 2022 - cell.com
We present a multiomic cell atlas of human lung development that combines single-cell RNA
and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging …

SARS-CoV-2 Omicron spike mediated immune escape and tropism shift

B Meng, IATM Ferreira, A Abdullahi, N Goonawardane… - BioRxiv, 2021 - biorxiv.org
Abstract The SARS-CoV-2 Omicron BA. 1 variant emerged in late 2021 and is characterised
by multiple spike mutations across all spike domains. Here we show that Omicron BA. 1 has …

Population-level integration of single-cell datasets enables multi-scale analysis across samples

C De Donno, S Hediyeh-Zadeh, AA Moinfar… - Nature …, 2023 - nature.com
The increasing generation of population-level single-cell atlases has the potential to link
sample metadata with cellular data. Constructing such references requires integration of …