Genetically encoded fluorescent biosensors illuminate the spatiotemporal regulation of signaling networks
Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …
Fluorescence lifetime measurements and biological imaging
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …
[LIVRE][B] FRET-Förster resonance energy transfer: from theory to applications
IL Medintz, N Hildebrandt - 2013 - books.google.com
FRET–Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed
primer on all aspects of the topic, this ready reference reflects the incredible expansion in …
primer on all aspects of the topic, this ready reference reflects the incredible expansion in …
An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching
Cyan fluorescent proteins (CFPs), such as Cerulean, are widely used as donor fluorophores
in Förster resonance energy transfer (FRET) experiments. Nonetheless, the most widely …
in Förster resonance energy transfer (FRET) experiments. Nonetheless, the most widely …
Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt
This protocol describes procedures for performing fluorescence resonance energy transfer
(FRET) microscopy analysis by three different methods: acceptor photobleaching, sensitized …
(FRET) microscopy analysis by three different methods: acceptor photobleaching, sensitized …
Activation of hormone-sensitive lipase requires two steps, protein phosphorylation and binding to the PAT-1 domain of lipid droplet coat proteins
H Wang, L Hu, K Dalen, H Dorward… - Journal of Biological …, 2009 - ASBMB
Lipolysis is an important metabolic pathway controlling energy homeostasis through
degradation of triglycerides stored in lipid droplets and release of fatty acids. Lipid droplets …
degradation of triglycerides stored in lipid droplets and release of fatty acids. Lipid droplets …
Fluorescence lifetime and polarization-resolved imaging in cell biology
JA Levitt, DR Matthews, SM Ameer-Beg… - Current opinion in …, 2009 - Elsevier
Fluorescence lifetime imaging (FLIM) and fluorescence polarization imaging are
complementary techniques that can be used to extract information about macromolecules …
complementary techniques that can be used to extract information about macromolecules …
eIF4F is a thermo-sensing regulatory node in the translational heat shock response
CD Altamirano, MK Kang, MA Jordan, T Borianne… - Molecular Cell, 2024 - cell.com
Heat-shocked cells prioritize the translation of heat shock (HS) mRNAs, but the underlying
mechanism is unclear. We report that HS in budding yeast induces the disassembly of the …
mechanism is unclear. We report that HS in budding yeast induces the disassembly of the …
FRET microscopy in 2010: the legacy of Theodor Förster on the 100th anniversary of his birth
The impact of great scientific insights can very often only be measured decades later.
Theodor Fçrster's theory of Fçrster (or fluorescence) resonance energy transfer (FRET) is …
Theodor Fçrster's theory of Fçrster (or fluorescence) resonance energy transfer (FRET) is …
A rationally enhanced red fluorescent protein expands the utility of FRET biosensors
Genetically encoded Förster Resonance Energy Transfer (FRET)-based biosensors are
powerful tools to illuminate spatiotemporal regulation of cell signaling in living cells, but the …
powerful tools to illuminate spatiotemporal regulation of cell signaling in living cells, but the …