Microfluidic platforms integrated with nano-sensors for point-of-care bioanalysis

H Tavakoli, S Mohammadi, X Li, G Fu, XJ Li - TrAC Trends in Analytical …, 2022 - Elsevier
Microfluidic technology provides a portable, cost-effective, and versatile tool for point-of-care
(POC) bioanalysis because of its associated advantages such as fast analysis, low volumes …

Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

H Zhang, L Cao, J Brodsky, I Gablech, F Xu, Z Li… - TrAC Trends in …, 2024 - Elsevier
This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …

High-throughput microfluidic systems accelerated by artificial intelligence for biomedical applications

J Zhou, J Dong, H Hou, L Huang, J Li - Lab on a Chip, 2024 - pubs.rsc.org
High-throughput microfluidic systems are widely used in biomedical fields for tasks like
disease detection, drug testing, and material discovery. Despite the great advances in …

Direct single-cell antimicrobial susceptibility testing of Escherichia coli in urine using a ready-to-use 3D microwell array chip

W Wu, G Cai, Y Liu, Y Suo, B Zhang, W **, Y Yu… - Lab on a Chip, 2023 - pubs.rsc.org
Empirical antibiotic therapies are prescribed for treating uncomplicated urinary tract
infections (UTIs) due to the long turnaround time of conventional antimicrobial susceptibility …

[HTML][HTML] Harnessing CRISPR/Cas Systems for DNA and RNA Detection: Principles, Techniques, and Challenges

H Son - Biosensors, 2024 - mdpi.com
The emergence of CRISPR/Cas systems has revolutionized the field of molecular
diagnostics with their high specificity and sensitivity. This review provides a comprehensive …

An optofluidic platform for cell-counting applications

MB Avci, SD Yasar, AE Cetin - Analytical Methods, 2023 - pubs.rsc.org
Cell-counting is critical for a wide range of applications, eg, life sciences, medicine, or
pharmacology. Hemocytometry is a classical method that requires manual counting of cells …

A one-stage deep learning based method for automatic analysis of droplet-based digital PCR images

Y Yao, S Zhao, Y Liang, F Hu, N Peng - Analyst, 2023 - pubs.rsc.org
Droplet-based dPCR offers many advantages over chip-based dPCR, such as lower
processing cost, higher droplet density, higher throughput, while requiring less sample …

SAM‐dPCR: Accurate and Generalist Nuclei Acid Quantification Leveraging the Zero‐Shot Segment Anything Model

Y Wei, S Luo, C Xu, Y Fu, Y Zhang, F Qu… - Advanced …, 2024 - Wiley Online Library
Digital PCR (dPCR) has transformed nucleic acid diagnostics by enabling the absolute
quantification of rare mutations and target sequences. However, traditional dPCR detection …

Deep‐qGFP: A Generalist Deep Learning Assisted Pipeline for Accurate Quantification of Green Fluorescent Protein Labeled Biological Samples in Microreactors

Y Wei, SMT Abbasi, N Mehmood, L Li, F Qu… - Small …, 2024 - Wiley Online Library
Absolute quantification of biological samples provides precise numerical expression levels,
enhancing accuracy, and performance for rare templates. Current methodologies, however …

Engineering innovative interfaces for point-of-care diagnostics

DT Burrow, JT Heggestad, DS Kinnamon… - Current Opinion in …, 2023 - Elsevier
The ongoing Coronavirus disease 2019 (COVID-19) pandemic illustrates the need for
sensitive and reliable tools to diagnose and monitor diseases. Traditional diagnostic …