Small RNAs, large networks: posttranscriptional regulons in gram-negative bacteria

K Papenfort, S Melamed - Annual review of microbiology, 2023 - annualreviews.org
Small regulatory RNA (sRNAs) are key mediators of posttranscriptional gene control in
bacteria. Assisted by RNA-binding proteins, a single sRNA often modulates the expression …

Bacterial RNA chaperones and chaperone-like riboregulators: behind the scenes of RNA-mediated regulation of cellular metabolism

K Katsuya-Gaviria, G Paris, T Dendooven… - RNA biology, 2022 - Taylor & Francis
In all domains of life, RNA chaperones safeguard and guide the fate of the cellular RNA
pool. RNA chaperones comprise structurally diverse proteins that ensure proper folding …

Dynamic refolding of OxyS sRNA by the Hfq RNA chaperone

H Cai, J Roca, YF Zhao, SA Woodson - Journal of molecular biology, 2022 - Elsevier
Abstract The Sm protein Hfq chaperones small non-coding RNAs (sRNAs) in bacteria,
facilitating sRNA regulation of target mRNAs. Hfq acts in part by remodeling the sRNA and …

Target recognition by RNase E RNA-binding domain AR2 drives sRNA decay in the absence of PNPase

D Sinha, NR De Lay - … of the National Academy of Sciences, 2022 - National Acad Sciences
The C-terminal domain (CTD) of the major endoribonuclease RNase E not only serves as a
scaffold for the central RNA decay machinery in gram-negative bacteria but also mediates …

Unraveling membrane perturbations caused by the bacterial riboregulator Hfq

F Turbant, J Waeytens, C Campidelli… - International Journal of …, 2022 - mdpi.com
Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism.
The protein notably regulates translation efficiency and RNA decay in Gram-negative …

Glutamine synthetase mRNA releases sRNA from its 3′ UTR to regulate carbon/nitrogen metabolic balance in Enterobacteriaceae

M Miyakoshi, T Morita, A Kobayashi, A Berger… - Elife, 2022 - elifesciences.org
Glutamine synthetase (GS) is the key enzyme of nitrogen assimilation induced under
nitrogen limiting conditions. The carbon skeleton of glutamate and glutamine, 2 …

TRS: a method for determining transcript termini from RNAtag-seq sequencing data

A Bar, L Argaman, M Eldar, H Margalit - Nature Communications, 2023 - nature.com
In bacteria, determination of the 3'termini of transcripts plays an essential role in regulation
of gene expression, affecting the functionality and stability of the transcript. Several …

An acetyltransferase moonlights as a regulator of the RNA binding repertoire of the RNA chaperone Hfq in Escherichia coli

X Luo, A Zhang, CH Tai, J Chen… - Proceedings of the …, 2023 - National Acad Sciences
Bacterial small RNAs (sRNAs) regulate gene expression by base-pairing with their target
mRNAs. In Escherichia coli and many other bacteria, this process is dependent on the RNA …

Intrinsically disordered interaction network in an RNA chaperone revealed by native mass spectrometry

SH Sarni, J Roca, C Du, M Jia, H Li… - Proceedings of the …, 2022 - National Acad Sciences
RNA-binding proteins contain intrinsically disordered regions whose functions in RNA
recognition are poorly understood. The RNA chaperone Hfq is a homohexamer that contains …

Transcriptome-scale analysis uncovers conserved residues in the hydrophobic core of the bacterial RNA chaperone Hfq required for small regulatory RNA stability

J McQuail, M Krepl, K Katsuya-Gaviria… - Nucleic Acids …, 2025 - academic.oup.com
The RNA chaperone Hfq plays crucial roles in bacterial gene expression and is a major
facilitator of small regulatory RNA (sRNA) action. The toroidal architecture of the Hfq …