Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the
predictable modification of genomes as they enable precise genome editing in heterologous …

Particle bombardment, or biolistics, has emerged as an excellent alternative approach for
plant genetic transformation which circumvents the limitations of Agrobacterium-mediated …

In rapidly adapting asexual populations, including many microbial pathogens and viruses,
numerous mutant lineages often compete for dominance within the population,,,–. These …

Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have
been randomly inserted in the genome. Consequently, develo** hybrids with multiple …

Transient expression of CRISPR/Cas9 is an effective approach for limiting its activities and
improving its precision in genome editing. Here, we describe the heat‐shock‐inducible …

Nannochloropsis oceanica CCMP1779 is an oleaginous heterokont microalga that is
particularly amenable to synthetic biology approaches. The underlying complex genetic …

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated
protein 9 nuclease (Cas9) system has emerged as the robust gene editing tool that functions …

Transgene integration in plants is based on illegitimate recombination between non-
homologous sequences. The low control of integration site and number of (trans/cis) gene …

Efforts to increase genetic gains in breeding programs of flowering plants depend on making
genetic crosses. Time to flowering, which can take months to decades depending on the …

Over the past two decades, new technologies enabling targeted modification of plant
genomes have been developed. Among these are zinc-finger nucleases (ZFNs) which are …