A retrospective analysis of P. falciparum drug resistance markers detects an early (2016/17) high prevalence of the k13 C469Y mutation in asymptomatic infections in …

R Ogwang, V Osoti, K Wamae, L Ndwiga… - Antimicrobial Agents …, 2024 - journals.asm.org
The emergence of drug-resistant Plasmodium falciparum parasites in sub-Saharan Africa
will substantially challenge malaria control. Here, we evaluated the frequency of common …

Seasonal malaria chemoprevention in a context of high presumed sulfadoxine-pyrimethamine resistance: malaria morbidity and molecular drug resistance profiles in …

I Molina-De la Fuente, MJ Sagrado Benito, E Lasry… - Malaria Journal, 2023 - Springer
Background Seasonal malaria chemoprevention (SMC) using sulfadoxine-pyrimethamine
plus amodiaquine (SP-AQ), is a community-based malaria preventive strategy commonly …

Molecular surveillance of Kelch 13 polymorphisms in Plasmodium falciparum isolates from Kenya and Ethiopia

B Jeang, D Zhong, MC Lee, H Atieli, D Yewhalaw… - Malaria Journal, 2024 - Springer
Background Timely molecular surveillance of Plasmodium falciparum kelch 13 (k13) gene
mutations is essential for monitoring the emergence and stemming the spread of artemisinin …

Imported malaria into Australia: surveillance insights and opportunities

A Sohail, A Barry, S Auburn, Q Cheng… - Journal of Travel …, 2024 - academic.oup.com
Background Malaria continues to pose a significant burden in endemic countries, many of
which lack access to molecular surveillance. Insights from malaria cases in travellers …

Detection of low frequency artemisinin resistance mutations, C469Y, P553L and A675V, and fixed antifolate resistance mutations in asymptomatic primary school …

V Osoti, K Wamae, L Ndwiga, PM Gichuki… - BMC Infectious …, 2025 - Springer
Background To understand the emergence and spread of drug-resistant parasites in malaria-
endemic areas, accurate assessment and monitoring of antimalarial drug resistance …

ONT sequencing identifies a high prevalence of crt sensitive, triple mutant dhfr and single mutant dhps parasites within an ANC population in Nigeria

AJ Adegbola, L Ndwiga, K Wamae, V Osoti… - Frontiers in …, 2024 - frontiersin.org
Background Malaria in pregnancy is a major public health issue, particularly among
vulnerable populations in malaria-endemic sub-Saharan African countries. To mitigate its …

Low frequency of Plasmodium falciparum hrp2/3 deletions from symptomatic infections at a primary healthcare facility in Kilifi, Kenya

D Okanda, L Ndwiga, V Osoti, N Achieng… - Frontiers in …, 2023 - frontiersin.org
There is a growing concern for malaria control in the Horn of Africa region due to the spread
and rise in the frequency of Plasmodium falciparum Histidine-rich Protein (hrp) 2 and 3 …

Detection of Low-frequency Artemisinin resistance mutations C469Y, P553L and A675V in asymptomatic primary school children in Kenya

V Osoti, K Wamae, L Ndwiga, PM Gichuki, C Okoyo… - 2024 - researchsquare.com
Background: To understand the emergence and spread of drug-resistant parasites in
malaria-endemic areas, accurate assessment and monitoring of antimalarial drug resistance …

Analytic optimization of Plasmodium falciparum marker gene haplotype recovery from amplicon deep sequencing of complex mixtures

Z Lapp, E Freedman, K Huang… - PLOS Global Public …, 2024 - journals.plos.org
Molecular epidemiologic studies of malaria parasites and other pathogens commonly
employ amplicon deep sequencing (AmpSeq) of marker genes derived from dried blood …

Molecular surveillance of artemisinin combination therapy resistance markers in Kelch13 gene of plasmodium falciparum isolates collected from Yobe State Nigeria

AA Zubair, GA Ajibade, AA Haroun… - Avicenna Journal of …, 2024 - ajcmi.umsha.ac.ir
Background: Despite the discovery of artemisinin and artemisinin combination therapies
(ACTs), resistance emerged due to single-nucleotide polymorphisms (SNPs) at the Kelch13 …