From hairballs to hypotheses–biological insights from microbial networks

L Röttjers, K Faust - FEMS microbiology reviews, 2018 - academic.oup.com
Microbial networks are an increasingly popular tool to investigate microbial community
structure, as they integrate multiple types of information and may represent systems-level …

The use of next generation sequencing for improving food safety: Translation into practice

B Jagadeesan, P Gerner-Smidt, MW Allard, S Leuillet… - Food …, 2019 - Elsevier
Abstract Next Generation Sequencing (NGS) combined with powerful bioinformatic
approaches are revolutionising food microbiology. Whole genome sequencing (WGS) of …

Primer, pipelines, parameters: issues in 16S rRNA gene sequencing

I Abellan-Schneyder, MS Matchado, S Reitmeier… - Msphere, 2021 - Am Soc Microbiol
Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies
investigating microbiomes. However, comparative studies on differences in procedures are …

Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2's q2-feature-classifier plugin

NA Bokulich, BD Kaehler, JR Rideout, M Dillon… - Microbiome, 2018 - Springer
Background Taxonomic classification of marker-gene sequences is an important step in
microbiome analysis. Results We present q2-feature-classifier (https://github. com/qiime2/q2 …

Deblur rapidly resolves single-nucleotide community sequence patterns

A Amir, D McDonald, JA Navas-Molina, E Kopylova… - …, 2017 - Am Soc Microbiol
High-throughput sequencing of 16S ribosomal RNA gene amplicons has facilitated
understanding of complex microbial communities, but the inherent noise in PCR and DNA …

Sequence clustering in bioinformatics: an empirical study

Q Zou, G Lin, X Jiang, X Liu, X Zeng - Briefings in bioinformatics, 2020 - academic.oup.com
Sequence clustering is a basic bioinformatics task that is attracting renewed attention with
the development of metagenomics and microbiomics. The latest sequencing techniques …

Microbiome diversity and dysbiosis in aquaculture

S Infante‐Villamil, R Huerlimann… - Reviews in …, 2021 - Wiley Online Library
With the continuous growth of the human population and associated need for high‐quality
protein, the aquaculture sector will be required to increase significantly in productivity. This …

Controlling for contaminants in low-biomass 16S rRNA gene sequencing experiments

L Karstens, M Asquith, S Davin, D Fair, WT Gregory… - …, 2019 - Am Soc Microbiol
Microbial communities are commonly studied using culture-independent methods, such as
16S rRNA gene sequencing. However, one challenge in accurately characterizing microbial …

Metagenome-assembled genome binning methods with short reads disproportionately fail for plasmids and genomic islands

F Maguire, B Jia, KL Gray, WYV Lau… - Microbial …, 2020 - microbiologyresearch.org
Metagenomic methods enable the simultaneous characterization of microbial communities
without time-consuming and bias-inducing culturing. Metagenome-assembled genome …