Lipoxygenases (LOXs) are enzymes that catalyze dioxygenation of polyunsaturated fatty
acids into fatty acid hydroperoxides. The formed fatty acid hydroperoxides are of interest as …

Streptomyces mobaraenesis transglutaminase can catalyze the cross-linking of proteins,
which has been widely used in food processing. In this study, we rationally modified flexible …

Highlights•Microorganisms and enzymes generate volatile flavours on the industrial
scale.•Side-streams of food processing provide an abundant source of flavour precursors.• …

Pseudomonas aeruginosa is an opportunistic pathogen that can cause nosocomial and
chronic infections in immunocompromised patients. P. aeruginosa secretes a lipoxygenase …

In this study, the bacterial lipoxygenase (LOX) gene from Pseudomonas aeruginosa
ATCC27853 (pse-LOX) was cloned, sequenced and heterologous expressed in Escherichia …

Cellulases have been used to extract bioactive ingredients from medical plants; however,
the poor enzymatic properties of current cellulases significantly limit their application. Two …

Background High thermostability is required for trypsin to have wider industrial applications.
Target mutagenesis at flexible regions has been proved to be an efficient protein …

In order to understand the role of N1 domain (1–257 aa) in the amylopullulanase (gt-apu) of
the extremely thermophilic bacterium Geobacillus thermoleovorans NP33, N1 deletion …

Abstract Pullulanase (EC 3.2. 1.41) is a well-known starch-debranching enzyme. Enhancing
the thermostability of Pullulanase is required for industrial application. In this study, we used …

Besides the catalytic ability, many enzymes contain conserved domains to perform some
other physiological functions. However, sometimes these conserved domains were …