Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

A review of super-resolution single-molecule localization microscopy cluster analysis and quantification methods

IM Khater, IR Nabi, G Hamarneh - Patterns, 2020 - cell.com
Single-molecule localization microscopy (SMLM) is a relatively new imaging modality,
winning the 2014 Nobel Prize in Chemistry, and considered as one of the key super …

Membrane phase separation drives responsive assembly of receptor signaling domains

SA Shelby, I Castello-Serrano, KC Wisser… - Nature Chemical …, 2023 - nature.com
Plasma membrane heterogeneity has been tied to a litany of cellular functions and is often
explained by analogy to membrane phase separation; however, models based on phase …

A trans-synaptic nanocolumn aligns neurotransmitter release to receptors

AH Tang, H Chen, TP Li, SR Metzbower… - Nature, 2016 - nature.com
Synaptic transmission is maintained by a delicate, sub-synaptic molecular architecture, and
even mild alterations in synapse structure drive functional changes during experience …

Measuring image resolution in optical nanoscopy

RPJ Nieuwenhuizen, KA Lidke, M Bates, DL Puig… - Nature …, 2013 - nature.com
Resolution in optical nanoscopy (or super-resolution microscopy) depends on the
localization uncertainty and density of single fluorescent labels and on the sample's spatial …

Phosphatidylserine vesicles enable efficient en bloc transmission of enteroviruses

YH Chen, WL Du, MC Hagemeijer, PM Takvorian… - Cell, 2015 - cell.com
A central paradigm within virology is that each viral particle largely behaves as an
independent infectious unit. Here, we demonstrate that clusters of enteroviral particles are …

Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors

F Pessina, F Giavazzi, Y Yin, U Gioia, V Vitelli… - Nature cell …, 2019 - nature.com
Damage-induced long non-coding RNAs (dilncRNA) synthesized at DNA double-strand
breaks (DSBs) by RNA polymerase II are necessary for DNA-damage-response (DDR) focus …

Force loading explains spatial sensing of ligands by cells

R Oria, T Wiegand, J Escribano, A Elosegui-Artola… - Nature, 2017 - nature.com
Cells can sense the density and distribution of extracellular matrix (ECM) molecules by
means of individual integrin proteins and larger, integrin-containing adhesion complexes …

ecDNA hubs drive cooperative intermolecular oncogene expression

KL Hung, KE Yost, L **e, Q Shi, K Helmsauer… - Nature, 2021 - nature.com
Extrachromosomal DNA (ecDNA) is prevalent in human cancers and mediates high
expression of oncogenes through gene amplification and altered gene regulation. Gene …

CDK7 inhibition potentiates genome instability triggering anti-tumor immunity in small cell lung cancer

H Zhang, CL Christensen, R Dries, MG Oser, J Deng… - Cancer cell, 2020 - cell.com
Summary Cyclin-dependent kinase 7 (CDK7) is a central regulator of the cell cycle and gene
transcription. However, little is known about its impact on genomic instability and cancer …