Development and Applications of D‐Amino Acid Derivatives‐based Metabolic Labeling of Bacterial Peptidoglycan

Y Zheng, X Zhu, M Jiang, F Cao, Q You… - Angewandte …, 2024 - Wiley Online Library
Peptidoglycan, an essential component within the cell walls of virtually all bacteria, is
composed of glycan strands linked by stem peptides that contain D‐amino acids. The …

Minimalist Tetrazine N-Acetyl Muramic Acid Probes for Rapid and Efficient Labeling of Commensal and Pathogenic Peptidoglycans in Living Bacterial Culture and …

AS Hillman, SN Hyland, KA Wodzanowski… - Journal of the …, 2024 - ACS Publications
N-Acetyl muramic acid (NAM) probes containing alkyne or azide groups are commonly used
to investigate aspects of cell wall synthesis because of their small size and ability to …

Bioorthogonal Radiolabeling of Azide-Modified Bacteria Using [18F]FB-sulfo-DBCO

AA Alanizi, AM Sorlin, MFL Parker… - Bioconjugate …, 2024 - ACS Publications
Purpose: This study was motivated by the need for better positron emission tomography
(PET)-compatible tools to image bacterial infection. Our previous efforts have targeted …

Advancing Spectrally‐Resolved Single Molecule Localization Microscopy with Deep Learning

H Manko, Y Mély, J Godet - Small, 2023 - Wiley Online Library
Spectrally‐resolved single molecule localization microscopy (srSMLM) is a recent technique
enriching single molecule localization microscopy with the simultaneous recording of …

Fluorescent d-amino Acid-Based Approach Enabling Fast and Reliable Measure of Antibiotic Susceptibility in Bacterial Cells

B Walenkiewicz, MS VanNieuwenhze - ACS Chemical Biology, 2024 - ACS Publications
The threat of multidrug-resistant bacteria has been increasing steadily in the past century,
posing a major health risk (Organización Mundial de la Salud. Directrices Sobre …

Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection

HM Betts, JC Luckett, PJ Hill - Molecular Imaging and Biology, 2024 - Springer
Purpose There is currently no ideal radiotracer for imaging bacterial infections.
Radiolabelled d-amino acids are promising candidates because they are actively …

A dual covalent binder for labelling and inhibiting serine and metallo-carbapenemases

C Chen, Y Xu, P Oelschlaeger, J Brem, L Liu… - Chemical …, 2023 - pubs.rsc.org
The continuous emergence of multi-drug resistant pathogens co-expressing serine and
metallo-carbapenemases seriously threatens the efficacy of carbapenem. Here, we report …

To click or not to click for short pulse-labeling of the bacterial cell wall

M Baudoin, A Chouquet, M Nguyen, A Zapun… - RSC …, 2024 - pubs.rsc.org
A method of choice to study the spatio-temporal dynamics of bacterial cell growth and
division is to analyze the localization of cell wall synthesis regions by fluorescence …

Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids

C Zhang, S Manley - The Bacterial Cell Wall: Methods and Protocols, 2023 - Springer
Fluorescent D-amino acids (FDAAs) enable in situ visualization of bacterial cell wall
synthesis via their incorporation into peptidoglycan (PG) crosslinks. When combined with …

An unsupervised deep learning framework encodes super-resolved image features to decode bacterial cell cycle

J Griffié, C Zhang, J Denereaz, TA Pham, G Weissbart… - bioRxiv, 2024 - biorxiv.org
Super-resolution microscopy can resolve cellular features at the nanoscale. However,
increased spatial resolution comes with increased phototoxicity, and reduced temporal …