CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

The proximal tubule is a key regulator of kidney function and glucose metabolism. Diabetic
kidney disease leads to proximal tubule injury and changes in chromatin accessibility that …

Epigenome editing has rapidly evolved in recent years, with diverse applications that
include elucidating gene regulation mechanisms, annotating coding and noncoding …

Cys2His2 zinc finger (ZF) domains engineered to bind specific target sequences in the
genome provide an effective strategy for programmable regulation of gene expression, with …

CRISPR interference (CRISPRi) enables programmable, reversible, and titratable
repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated …

Kidney failure, the decrease of kidney function below a threshold necessary to support life, is
a major cause of morbidity and mortality. We performed a genome-wide association study …

The CRISPR-Cas system is commonly known for its ability to cleave DNA in a
programmable manner, which has democratized gene editing and facilitated recent …

Transcription is orchestrated by thousands of transcription factors (TFs) and chromatin-
associated proteins, but how these are causally connected to transcriptional activation is …