Conformational analysis of complex protein states by hydrogen/deuterium exchange mass spectrometry (HDX-MS): Challenges and emerging solutions

E Trabjerg, ZE Nazari, KD Rand - TrAC Trends in Analytical Chemistry, 2018 - Elsevier
Hydrogen/deuterium exchange mass spectrometry (HDX-MS) has within the last 25 years
evolved to become a well-established technique for analysing the solution-phase …

Structural basis of the pore-forming toxin/membrane interaction

Y Li, Y Li, HM Mengist, C Shi, C Zhang, B Wang, T Li… - Toxins, 2021 - mdpi.com
With the rapid growth of antibiotic-resistant bacteria, it is urgent to develop alternative
therapeutic strategies. Pore-forming toxins (PFTs) belong to the largest family of virulence …

Structure and allosteric inhibition of excitatory amino acid transporter 1

JC Canul-Tec, R Assal, E Cirri, P Legrand, S Brier… - Nature, 2017 - nature.com
Human members of the solute carrier 1 (SLC1) family of transporters take up excitatory
neurotransmitters in the brain and amino acids in peripheral organs. Dysregulation of the …

Calcium-driven folding of RTX domain β-rolls ratchets translocation of RTX proteins through type I secretion ducts

L Bumba, J Masin, P Macek, T Wald, L Motlova… - Molecular cell, 2016 - cell.com
Calcium-binding RTX proteins are equipped with C-terminal secretion signals and
translocate from the Ca 2+-depleted cytosol of Gram-negative bacteria directly into the Ca …

Structure of the calcium-dependent type 2 secretion pseudopilus

A Lopez-Castilla, JL Thomassin, B Bardiaux… - Nature …, 2017 - nature.com
Many Gram-negative bacteria use type 2 secretion systems (T2SSs) to secrete proteins
involved in virulence and adaptation. Transport of folded proteins via T2SS nanomachines …

An arsenal of methods for the experimental characterization of intrinsically disordered proteins–how to choose and combine them?

A Schramm, C Bignon, S Brocca, R Grandori… - Archives of biochemistry …, 2019 - Elsevier
In this review, we detail the most common experimental approaches to assess and
characterize protein intrinsic structural disorder, with the notable exception of NMR and EPR …

Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices

YC Shin, JH Lee, MJ Kim, SW Hong, B Kim… - Journal of Biological …, 2015 - Springer
Background In the field of biomedical engineering, many studies have focused on the
possible applications of graphene and related nanomaterials due to their potential for use as …

MEMHDX: an interactive tool to expedite the statistical validation and visualization of large HDX-MS datasets

V Hourdel, S Volant, DP O'Brien, A Chenal… - …, 2016 - academic.oup.com
Motivation: With the continued improvement of requisite mass spectrometers and UHPLC
systems, Hydrogen/Deuterium eXchange Mass Spectrometry (HDX-MS) workflows are …

Small-angle scattering studies of intrinsically disordered proteins and their complexes

TN Cordeiro, F Herranz-Trillo, A Urbanek… - Current opinion in …, 2017 - Elsevier
Highlights•Despite the absence of permanent structure, IDPs perform crucial functions in
biology.•Structural studies of IDPs require the combination of SAS with computation.• …

Structural disorder and induced folding within two cereal, ABA stress and ripening (ASR) proteins

K Hamdi, E Salladini, DP O'Brien, S Brier, A Chenal… - Scientific reports, 2017 - nature.com
A bscisic acid (ABA), s tress and r ipening (ASR) proteins are plant-specific proteins involved
in plant response to multiple abiotic stresses. We previously isolated the ASR genes and …