Thermal shift assay for protein–ligand dissociation constant determination

V Petrauskas, E Kazlauskas, M Gedgaudas… - TrAC Trends in …, 2024 - Elsevier
Accurate determination of protein-ligand affinity is essential for drug design when new
compounds should be ranked according to their dissociation constants K d. The thermal shift …

Enzyme Databases in the Era of Omics and Artificial Intelligence

U Prešern, M Goličnik - International Journal of Molecular Sciences, 2023 - mdpi.com
Enzyme research is important for the development of various scientific fields such as
medicine and biotechnology. Enzyme databases facilitate this research by providing a wide …

Comprehensive analysis of resorcinyl-imidazole Hsp90 inhibitor design

M Gedgaudas, P Kaziukonytė, V Kairys… - European Journal of …, 2024 - Elsevier
Human Hsp90 chaperones are implicated in various aspects of cancer. Due to this, Hsp90
has been explored as potential target in cancer treatment. Initial attempts to use Hsp90 …

[HTML][HTML] From X-ray crystallographic structure to intrinsic thermodynamics of protein–ligand binding using carbonic anhydrase isozymes as a model system

V Paketurytė-Latvė, A Smirnov, E Manakova… - IUCrJ, 2024 - journals.iucr.org
Carbonic anhydrase (CA) was among the first proteins whose X-ray crystal structure was
solved to atomic resolution. CA proteins have essentially the same fold and similar active …

The interoperability of crystallographic data and databases

A Brink, I Bruno, JR Helliwell, B McMahon - IUCrJ, 2024 - journals.iucr.org
Interoperability of crystallographic data with other disciplines is essential for the smooth and
rapid progress of structure-based science in the computer age. Within crystallography and …

Targeted anticancer pre-vinylsulfone covalent inhibitors of carbonic anhydrase IX

A Vaškevičius, D Baronas, J Leitans, A Kvietkauskaitė… - eLife, 2024 - elifesciences.org
We designed novel pre-drug compounds that transform into an active form that covalently
modifies particular His residue in the active site, a difficult task to achieve, and applied to …

Carbonic anhydrase IX inhibition as a path to treat neuroblastoma

A Petrosiute, A Zakšauskas… - British Journal of …, 2024 - Wiley Online Library
Abstract Background and Purpose Tumour hypoxia frequently presents a major challenge in
the treatment of neuroblastoma (NBL). The neuroblastoma cells produce carbonic …

Inhibitor binding to metal-substituted metalloenzyme: Sulfonamide affinity for carbonic anhydrase IX

D Baronas, B Knašienė, A Mickevičiūtė… - Journal of Inorganic …, 2024 - Elsevier
Transition metal ions are structural and catalytic cofactors of many proteins including human
carbonic anhydrase (CA), a Zn-dependent hydrolase. Sulfonamide inhibitors of CA …

From X-ray crystallographic structure to intrinsic thermodynamics of protein–ligand binding using carbonic anhydrase isozymes as a model system

V Paketurytė-Latvė, A Smirnov, E Manakova… - IUCrJ, 2024 - journals.iucr.org
Carbonic anhydrase (CA) was among the first proteins whose X-ray crystal structure was
solved to atomic resolution. CA proteins have essentially the same fold and similar active …

[HTML][HTML] Databases of ligand-binding pockets and protein-ligand interactions

KA Carpenter, RB Altman - Computational and Structural Biotechnology …, 2024 - Elsevier
Many research groups and institutions have created a variety of databases curating
experimental and predicted data related to protein-ligand binding. The landscape of …