Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins
In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …
Expanding and reprogramming the genetic code of cells and animals
JW Chin - Annual review of biochemistry, 2014 - annualreviews.org
Genetic code expansion and reprogramming enable the site-specific incorporation of
diverse designer amino acids into proteins produced in cells and animals. Recent advances …
diverse designer amino acids into proteins produced in cells and animals. Recent advances …
Structural basis for aminoglycoside inhibition of bacterial ribosome recycling
MA Borovinskaya, RD Pai, W Zhang… - Nature structural & …, 2007 - nature.com
Aminoglycosides are widely used antibiotics that cause messenger RNA decoding errors,
block mRNA and transfer RNA translocation, and inhibit ribosome recycling. Ribosome …
block mRNA and transfer RNA translocation, and inhibit ribosome recycling. Ribosome …
Tuning response curves for synthetic biology
J Ang, E Harris, BJ Hussey, R Kil… - ACS synthetic …, 2013 - ACS Publications
Synthetic biology may be viewed as an effort to establish, formalize, and develop an
engineering discipline in the context of biological systems. The ability to tune the properties …
engineering discipline in the context of biological systems. The ability to tune the properties …
Maturation and degradation of ribosomal RNA in bacteria
MP Deutscher - Progress in molecular biology and translational …, 2009 - Elsevier
Ribosomal RNAs are the major components of ribosomes and are responsible for their
catalytic activity. The three bacterial rRNAs (16S, 23S, and 5S) are cotranscribed as a single …
catalytic activity. The three bacterial rRNAs (16S, 23S, and 5S) are cotranscribed as a single …
Synthesis of orthogonal transcription-translation networks
W An, JW Chin - Proceedings of the National Academy of …, 2009 - National Acad Sciences
Orthogonal, parallel and independent, systems are one key foundation for synthetic biology.
The synthesis of orthogonal systems that are uncoupled from evolutionary constraints, and …
The synthesis of orthogonal systems that are uncoupled from evolutionary constraints, and …
Reprogramming the genetic code: from triplet to quadruplet codes
The genetic code of cells is near‐universally triplet, and since many ribosomal mutations are
lethal, changing the cellular ribosome to read nontriplet codes is challenging. Herein we …
lethal, changing the cellular ribosome to read nontriplet codes is challenging. Herein we …
[HTML][HTML] The presence of highly disruptive 16S rRNA mutations in clinical samples indicates a wider role for mutations of the mitochondrial ribosome in human disease
Mitochondrial DNA mutations are well recognized as an important cause of disease, with
over two hundred variants in the protein encoding and mt-tRNA genes associated with …
over two hundred variants in the protein encoding and mt-tRNA genes associated with …
Incorporation of unnatural amino acids for synthetic biology
N Voloshchuk, JK Montclare - Molecular Biosystems, 2010 - pubs.rsc.org
The challenge of synthetic biology lies in the construction of artificial cellular systems. This
requires the development of modular “parts” that can be integrated into living systems to …
requires the development of modular “parts” that can be integrated into living systems to …
Biosynthesis and genetic incorporation of 3, 4-Dihydroxy-L-phenylalanine into proteins in Escherichia Coli
While 20 canonical amino acids are used by most organisms for protein synthesis, the
creation of cells that can use noncanonical amino acids (ncAAs) as additional protein …
creation of cells that can use noncanonical amino acids (ncAAs) as additional protein …