Genome engineering for crop improvement and future agriculture
C Gao - Cell, 2021 - cell.com
Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …
changing climate conditions. Genome editing is set to revolutionize plant breeding and …
Applications of CRISPR–Cas in agriculture and plant biotechnology
The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …
molecular biology beyond all expectations. Characterized by robustness and high target …
Plants' physio-biochemical and phyto-hormonal responses to alleviate the adverse effects of drought stress: A comprehensive review
Water, a necessary component of cell protoplasm, plays an essential role in supporting life
on Earth; nevertheless, extreme changes in climatic conditions limit water availability …
on Earth; nevertheless, extreme changes in climatic conditions limit water availability …
CRISPR/Cas9 therapeutics: progress and prospects
T Li, Y Yang, H Qi, W Cui, L Zhang, X Fu, X He… - … and Targeted Therapy, 2023 - nature.com
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …
Prime genome editing in rice and wheat
Q Lin, Y Zong, C Xue, S Wang, S **, Z Zhu… - Nature …, 2020 - nature.com
Prime editors, which are CRISPR–Cas9 nickase (H840A)–reverse transcriptase fusions
programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells …
programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells …
Major impacts of widespread structural variation on gene expression and crop improvement in tomato
Structural variants (SVs) underlie important crop improvement and domestication traits.
However, resolving the extent, diversity, and quantitative impact of SVs has been …
However, resolving the extent, diversity, and quantitative impact of SVs has been …
Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …
decade and holds great promise for the treatment of human genetic diseases. Here we …
An engineered prime editor with enhanced editing efficiency in plants
Prime editing is a versatile genome-editing technology, but it suffers from low editing
efficiency. In the present study, we introduce optimized prime editors with substantially …
efficiency. In the present study, we introduce optimized prime editors with substantially …
High-efficiency prime editing with optimized, paired pegRNAs in plants
Prime editing (PE) applications are limited by low editing efficiency. Here we show that
designing prime binding sites with a melting temperature of 30° C leads to optimal …
designing prime binding sites with a melting temperature of 30° C leads to optimal …
Conventional and molecular techniques from simple breeding to speed breeding in crop plants: recent advances and future outlook
In most crop breeding programs, the rate of yield increment is insufficient to cope with the
increased food demand caused by a rapidly expanding global population. In plant breeding …
increased food demand caused by a rapidly expanding global population. In plant breeding …