Actin visualization at a glance

M Melak, M Plessner, R Grosse - Journal of cell science, 2017 - journals.biologists.com
Actin functions in a multitude of cellular processes owing to its ability to polymerize into
filaments, which can be further organized into higher-order structures by an array of actin …

Mechanical properties of single cells: Measurement methods and applications

Y Hao, S Cheng, Y Tanaka, Y Hosokawa… - Biotechnology …, 2020 - Elsevier
Cell mechanical properties, eg elastic and shear modulus, play vital roles in cell activities
and functions, such as cell growth, cell division, cell motion, and cell adhesion …

Control of synapse structure and function by actin and its regulators

JE Gentile, MG Carrizales, AJ Koleske - Cells, 2022 - mdpi.com
Neurons transmit and receive information at specialized junctions called synapses.
Excitatory synapses form at the junction between a presynaptic axon terminal and a …

Lifeact-TagGFP2 alters F-actin organization, cellular morphology and biophysical behaviour

LR Flores, MC Keeling, X Zhang, K Sliogeryte… - Scientific reports, 2019 - nature.com
Live-imaging techniques are at the forefront of biology research to explore behaviour and
function from sub-cellular to whole organism scales. These methods rely on intracellular …

Advances in micropipette aspiration: applications in cell biomechanics, models, and extended studies

B González-Bermúdez, GV Guinea, GR Plaza - Biophysical Journal, 2019 - cell.com
With five decades of sustained application, micropipette aspiration has enabled a wide
range of biomechanical studies in the field of cell mechanics. Here, we provide an update on …

Biophysical approaches for applying and measuring biological forces

W Sun, X Gao, H Lei, W Wang, Y Cao - Advanced Science, 2022 - Wiley Online Library
Over the past decades, increasing evidence has indicated that mechanical loads can
regulate the morphogenesis, proliferation, migration, and apoptosis of living cells …

Structure of the Lifeact–F-actin complex

A Belyy, F Merino, O Sitsel, S Raunser - PLoS biology, 2020 - journals.plos.org
Lifeact is a short actin-binding peptide that is used to visualize filamentous actin (F-actin)
structures in live eukaryotic cells using fluorescence microscopy. However, this popular …

Gliding motility of the diatom Craspedostauros australis coincides with the intracellular movement of raphid-specific myosins

MG Davutoglu, VF Geyer, L Niese… - Communications …, 2024 - nature.com
Raphid diatoms are one of the few eukaryotes capable of gliding motility, which is
remarkably fast and allows for quasi-instantaneous directional reversals. Besides other …

Anisotropy vs isotropy in living cell indentation with AFM

YM Efremov, M Velay-Lizancos, CJ Weaver… - Scientific reports, 2019 - nature.com
The measurement of local mechanical properties of living cells by nano/micro indentation
relies on the foundational assumption of locally isotropic cellular deformation. As a …

Tug-of-war between actomyosin-driven antagonistic forces determines the positioning symmetry in cell-sized confinement

R Sakamoto, M Tanabe, T Hiraiwa, K Suzuki… - Nature …, 2020 - nature.com
Symmetric or asymmetric positioning of intracellular structures including the nucleus and
mitotic spindle steers various biological processes such as cell migration, division, and …