Lateral flow test engineering and lessons learned from COVID-19

J Budd, BS Miller, NE Weckman, D Cherkaoui… - Nature Reviews …, 2023 - nature.com
The acceptability and feasibility of large-scale testing with lateral flow tests (LFTs) for clinical
and public health purposes has been demonstrated during the COVID-19 pandemic. LFTs …

Recent advances in point-of-care testing of COVID-19

S Lee, L Bi, H Chen, D Lin, R Mei, Y Wu… - Chemical Society …, 2023 - pubs.rsc.org
Advances in microfluidic device miniaturization and system integration contribute to the
development of portable, handheld, and smartphone-compatible devices. These …

A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs

H Yan, Y Wen, Z Tian, N Hart, S Han… - Nature Biomedical …, 2023 - nature.com
The use of microRNAs as clinical cancer biomarkers is hindered by the absence of accurate,
fast and inexpensive assays for their detection in biofluids. Here we report a one-step and …

Recent progress of smartphone-assisted microfluidic sensors for point of care testing

G **ng, J Ai, N Wang, Q Pu - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
Point of care (POC) testing is of great importance in rapid diagnosis of disease, monitoring
environmental pollutants, food safety analysis and other fields, especially in resource-limited …

Nanoplasmonic amplification in microfluidics enables accelerated colorimetric quantification of nucleic acid biomarkers from pathogens

T AbdElFatah, M Jalali, SG Yedire, I I. Hosseini… - Nature …, 2023 - nature.com
Deployment of nucleic acid amplification assays for diagnosing pathogens in point-of-care
settings is a challenge due to lengthy preparatory steps. We present a molecular diagnostic …

Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes

R Ren, S Cai, X Fang, X Wang, Z Zhang… - Nature …, 2023 - nature.com
We report on single-molecule nanopore sensing combined with position-encoded DNA
molecular probes, with chemistry tuned to simultaneously identify various antigen proteins …

Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device

Y Liu, Y Yang, G Wang, D Wang, PL Shao… - Nature Biomedical …, 2023 - nature.com
Portable assays for the rapid identification of lineages of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the …

CRISPR-based biosensors for pathogenic biosafety

H Yang, R Ledesma-Amaro, H Gao, Y Ren… - Biosensors and …, 2023 - Elsevier
Pathogenic biosafety is a worldwide concern. Tools for analyzing pathogenic biosafety, that
are precise, rapid and field-deployable, are highly demanded. Recently developed …

A programmable DNAzyme for the sensitive detection of nucleic acids

C Shi, D Yang, X Ma, L Pan, Y Shao… - Angewandte Chemie …, 2024 - Wiley Online Library
Nucleic acids in biofluids are emerging biomarkers for the molecular diagnostics of
diseases, but their clinical use has been hindered by the lack of sensitive detection assays …

CRISPR‐Mediated Profiling of Viral RNA at Single‐Nucleotide Resolution

D Chen, W Huang, Y Zhang, B Chen… - Angewandte Chemie …, 2023 - Wiley Online Library
Mass pathogen screening is critical to preventing the outbreaks and spread of infectious
diseases. The large‐scale epidemic of COVID‐19 and the rapid mutation of the severe acute …