Small-molecule fluorophores manifest the ability of chemistry to solve problems in biology.
As we noted in a previous review (Lavis, LD; Raines, RT ACS Chem. Biol. 2008, 3, 142 …

Visualizing and manipulating the behavior of proteins is crucial to understanding the
physiology of the cell. Methods of biorthogonal protein labeling are important tools to attain …

The self-labeling protein tags (SLPs) HaloTag7, SNAP-tag, and CLIP-tag allow the covalent
labeling of fusion proteins with synthetic molecules for applications in bioimaging and …

Genetically encoded fluorescent tags are protein sequences that can be fused to a protein of
interest to render it fluorescent. These tags have revolutionized cell biology by allowing …

Naturally occurring fluorescent proteins (FPs) are the most widely used tools for tracking
cellular proteins and sensing cellular events. Here, we chemically evolved the self-labeling …

The field of directed evolution has progressed to the point where it is feasible to engineer
enzymes for unnatural substrates and reactions with catalytic efficiencies and regio …

Single-molecule imaging provides a powerful way to study biochemical processes in live
cells, yet it remains challenging to track single molecules while simultaneously detecting …

Recent years have seen a remarkable growth in the field of protein-based medical
treatments. Nevertheless, concerns have arisen regarding the cytotoxicity limitations, low …

The inefficient translocation of proteins across biological membranes limits their application
as potential therapeutics and research tools. In many cases, the translocation of a protein …

The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and
endocytosis, but its performance has not been duplicated in red fluorescent protein …