(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions–a review

CMH Ferreira, ISS Pinto, EV Soares, HMVM Soares - Rsc Advances, 2015 - pubs.rsc.org
The use of buffers to maintain the pH within a desired range is a very common practice in
chemical, biochemical and biological studies. Among them, zwitterionic N-substituted …

History and principles of conductive media for standard DNA electrophoresis

JR Brody, SE Kern - Analytical biochemistry, 2004 - Elsevier
DNA electrophoresis has been a dominant technique in molecular biology for 30 years. The
foundation for this common technique is based on a few simple electrochemical principles …

Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis

JR Brody, SE Kern - Biotechniques, 2004 - Taylor & Francis
Figure 1. Sodium boric acid (SB) performance in agarose DNA electrophoresis as compared
to commonly used conductive media. Gels comprised 1.2%(lanes 1− 4) and 0.7%(lanes 5 …

[KÖNYV][B] Handbook of biochemistry and molecular biology

RL Lundblad, F Macdonald - 2018 - books.google.com
Edited by renowned protein scientist and bestselling author Roger L. Lundblad, with the
assistance of Fiona M. Macdonald of CRC Press, this fifth edition of the Handbook of …

Not only pH. Specific buffer effects in biological systems

A Salis, M Monduzzi - Current Opinion in Colloid & Interface Science, 2016 - Elsevier
The aim of this work is to overview the specific effect of pH buffers in biological systems. The
pH of a buffer solution changes only slightly when a small amount of a strong acid or bases …

Optimizing the culture environment in the IVF laboratory: impact of pH and buffer capacity on gamete and embryo quality

JE Swain - Reproductive biomedicine online, 2010 - Elsevier
Supplying and maintaining appropriate culture conditions is critical to minimize stress
imposed upon gametes and embryos and to optimize the in-vitro environment. One …

Biological pH buffers in IVF: help or hindrance to success

MA Will, NA Clark, JE Swain - Journal of assisted reproduction and …, 2011 - Springer
Purpose Minimizing environmental stress helps maintain cellular homeostasis and is a
crucial component in optimizing embryo development in vitro and resulting ART success …

Interactions of TRIS [tris (hydroxymethyl) aminomethane] and related buffers with peptide backbone: thermodynamic characterization

M Taha, MJ Lee - Physical Chemistry Chemical Physics, 2010 - pubs.rsc.org
In a situation which is far from ideal, many buffers have been found to be quite reactive,
besides maintaining their stable pH values. On the basis of apparent transfer free energies …

The melting curves of calf thymus-DNA are buffer specific

M Mura, C Carucci, FC Marincola, M Monduzzi… - Journal of Colloid and …, 2023 - Elsevier
The specific effects of salts (strong electrolytes) on biomolecular properties have been
investigated for more than a century. By contrast, the specific role of pH buffers (weak …

Embryo culture media for human IVF: which possibilities exist?

I Gruber, M Klein - Journal of the Turkish German …, 2011 - pmc.ncbi.nlm.nih.gov
The last three decades have seen considerable progress in the development of culture
media for ART and infertility treatment. Basic research on the metabolism of mammalian …