Abstract Develo** artificial “CO 2-sugar” platforms is meaningful for addressing
challenges posed by land scarcity and climate change to the supply of dietary sugar …

Enzyme-based synthetic chemistry provides a green way to synthesize industrially important
chemical scaffolds and provides incomparable substrate specificity and unmatched stereo …

Detoxication, or 'drug‐metabolizing', enzymes and drug transporters exhibit remarkable
substrate promiscuity and catalytic promiscuity. In contrast to substrate‐specific enzymes …

The abundance of recorded protein sequence data stands in contrast to the small number of
experimentally verified functional annotation. Here we screened a million-membered …

Naturally, haloacid dehalogenase superfamily phosphatases have been evolved with broad
substrate promiscuity; however, strong specificity to a particular substrate is required for …

Phase II drug metabolism inactivates xenobiotics and endobiotics through the addition of
either a glucuronic acid or sulfate moiety prior to excretion, often via the gastrointestinal tract …

Promiscuous enzymes can be recruited to serve new functions when a genetic or
environmental change makes catalysis of a novel reaction important for fitness or even …

We report a systematic combinatorial exploration of affinity enhancement of antibodies by
insertions and deletions (InDels). Transposon-based introduction of InDels via the method …

Directed evolution of enzymes toward improved catalytic performance has become a
powerful tool in protein engineering. To be effective, a directed evolution campaign requires …

The evolution of novel enzymes has fueled the diversification of life on earth for billions of
years. Insights into events that set the stage for the evolution of a new enzyme can be …